Please do not bookmark specific publications. The URL for each item changes each quarter as our Research Products database is refreshed. If you have a question regarding this publication, use the "Contact Us" feature above and include the product citation in your message.

Research Product

Selifonov, Sergey A., Jerome E. Gurst and Lawrence P. Wackett. 1995. Regioselective Dioxygenation of ortho-Trifluoromethylbenzoate by Pseudomonas aeruginosa 142: Evidence for 1,2-Dioxygenation as a Mechanism in ortho-Halobenzoate Dehalogenation. EPA/600/J-96/074. Biochem. Biophys. Res. Commun. 213(3):759-767. (ERL,GB X817).

Pseudomonas aeruginosa strain 142 oxidizes 2-halobenzoates via a multicomponent oxygenase (V.Romanov and R.P. Hausinger, J. Bacteriol., 1994, 176(11). The intermediacy of a highly unstable cis-diol in the reaction has been proposed. Direct evidence for this is currently lacking and the stereochemical course of the reaction cannot be inferred from previous studies. In this study, 2-trifluoromethylbenzoate was stoichiometrically oxidized by P. aeruginosa 142 to a chiral product identified as (-)2-trifluoromethyl-cis-1,2-dihydroxy-3,5-cyclohexadiene-1-carboxylic acid. These data rigorously establish a dioxygenative mechanism for 2-halobenzoate metabolism.

horizontal blue bar

[ ORD Home | NHEERL Home ]

Research Product

Local Navigation