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Training Modules
For the Long Term 2 Enhanced Surface Water Treatment Rule
On-line training modules have been developed to provide additional guidance and instruction to utility and laboratory personnel on LT2 sample collection and analysis. These training modules are meant to complement and expand on the guidance manuals developed for the Long Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR or LT2 rule).
The following training modules require the use of the Flash player plug-in. If you do not have Flash installed, you can visit Adobe's Web site to download and install the plug-in for your browser. Get the Flash player. 
- LT2 Training Module - Basic Microscopy (Opens in a pop-up window)
This module is designed to assist analysts in the proper use and maintenance of a microscope as used to detect Cryptosporidium and Giardia in Method 1623.
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On-line Sample Collection Training Module (Opens in a pop-up window)
This module is designed to help utility staff and others that will collect samples under the LT2 rule. The module details the sampling procedures for bulk sample collection and field filtration, and provides instruction on proper sample packing and shipping.
- On-line Microscopy Training Module (Opens in a pop-up window)
This module is designed to help analysts properly identify and characterize Cryptosporidium and Giardia using Method 1622/1623. The module includes characterization by FITC, DAPI, and DIC of positive control organisms and interfering organisms.
- Immunomagnetic Separation Training Module (Opens in a pop-up window)
This module details the immunomagnetic separation (IMS) procedure used in EPA Method 1622/1623 to separate Cryptosporidium oocysts and Giardia cysts from extraneous debris in a water sample.
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