Skip common site navigation and headers
United States Environmental Protection Agency
National Risk Management Research Laboratory
Begin Hierarchical Links EPA Home > Research & Development > National Risk Management Research Laboratory > Publications > EPA 625/R-92/013 End Hierarchical Links
EPA's Explorer's Club for Kids




Environmental Regulations and Technology: Control of Pathogens and Vector Attraction in Sewage Sludge
Revised July 2003

You will need Adobe Acrobat Reader to view Adobe PDF documents.
Click here to read About Portable Document Format Files.

Preliminary Pages (PDF, 1147 Kb, 8 pp)
Chapter 1 - Introduction (PDF, 641 Kb, 7 pp)
Chapter 2 - Sewage Sludge Pathogens (PDF, 1101 Kb, 12 pp)
Chapter 3 - Overview of Part 503 Subpart D Requirements, Their Applicability, and Related Requirements (PDF, 153 Kb, 6 pp)
Chapter 4 - Class A Pathogen Requirements (PDF, 170 Kb, 10 pp)
Chapter 5 - Class B Pathogen Requirements and Requirements for Domestic Septage Applied to Agricultural Land, a Forest, or a Reclamation Site (PDF, 678 Kb, 7 pp)
Chapter 6 - Processes to Significantly Reduce Pathogens (PDF, 1107 Kb, 8 pp)
Chapter 7 - Processes to Further Reduce Pathogens (PDF, 642 Kb, 7 pp)
Chapter 8 - Requiremetns for Reducing Vector Attraction (PDF, 615 Kb, 7 pp)
Chapter 9 - Sampling Procedures and Analytical Methods (PDF, 601 Kb, 11 pp)
Chapter 10 - Meeting the Quantitative Requirements of the Regulation (PDF, 605 Kb, 14 pp)
Chapter 11 - Role of EPA's Pathogen Equivalency Committee in Providing Guidance Under Part 503 (PDF, 652 Kb, 13 pp)
Chapter 12 - References and Additional Resources (PDF, 472 Kb, 4 pp)
Appendix A - EPA Regional and State Biosolids Coordinators and Listing of EPA Pathogen Equivalency Members (PDF, 436 Kb, 8 pp)
Chapter B - Subpart D of the Part 503 Regulation (PDF, 453 Kb, 5 pp)
Chapter C - Determination of Volatile Solids Reduction by Digestion (PDF, 473 Kb, 7 pp)
Chapter D - Guidance on Three Vector Attraction Reduction Tests (PDF, 98 Kb, 6 pp)
Chapter E - Determination of Residence Time for Anaerobic and Aerobic Digestion (PDF, 357 Kb, 4 pp)
Chapter F - Sample Preparation for Fecal Coliform Tests and Salmonella sp. Analysis (PDF, 366 Kb, 4 pp)
Chapter G - Kenner and Clark (1974) Analytical Method for Salmonella sp. Bacteria (PDF, 425 Kb, 9 pp)
Chapter H - Method for the Recovery and Assay of Total Culturable Viruses from Sludge (PDF, 355 Kb, 16 pp)
Chapter I - Test Method for Detecting, Enumerating, and Determining the Viability of Ascaris Ova in Sludge (PDF, 510 Kb, 7 pp)
Chapter J - The Biosolids Composting Process (PDF, 366 Kb, 6 pp)


This report is a primary reference for regional, state, and local regulatory authorities and their constituents. It describes the federal requirements concerning pathogens in sewage sludge and septage destined for land application or surface disposal, and it provides guidance for meeting those requirements. The document has undergone minor revisions from the 1999 edition, including minor editorial changes, an updated list of EPA and State biosolids coordinators, and minor updates to the procedures for analyzing bacteria, helminth ova, and enteroviruses.

Chapter 1 defines sewage sludge, biosolids and beneficial use, and provides an overview of the regulation governing sewage sludge. Chapter 2 provides basic information about pathogens and describes why pathogen control is required to protect public health and the environment, and Chapters 3 through 5 discuss the current federal requirements under Subpart D of Part 503. Chapters 6 and 7 review the different PFRP and PSRP processes, and Chapter 8 discusses vector attraction reduction issues. Chapters 9 and 10 summarize sampling and analysis protocols used to meet the quantitative requirements of Part 503. Chapter 11 outlines the process for applying for equivalency and discusses the kind of support EPA’s Pathogen Equivalency Committee can provide to permitting authorities. Chapter 12 lists general references and additional resources related to biosolids use; specific references related to particular topics are also included at the end of each chapter.

James Smith


Office of Research & Development | National Risk Management Research Laboratory

Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us