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Mueller, J.G., S.E. Lantz, R. Devereux, J.D. Berg and P.H. Pritchard. 1994. Studies on the Microbial Ecology of Polycyclic Aromatic Hydrocarbon Biodegradation. In: Bioremediation of Chlorinated and Polycyclic Aromatic Hydrocarbon Compounds. EPA/600/A-94/198. Robert E. Hinchee et al., Editor. Lewis Publishers, Boca Raton, FL. Pp. 218-230. (ERL,GB 831). (Avail. from NTIS, Springfield, VA: PB95-123006)
Soils with known history of exposure to polycyclic aromatic hydrocarbons (PAHs) were collected from Norway, Germany and the United States and screened for the presence of PAH-degrading bacteria. Purified PAH-degrading isolates were characterized by fatty acid profile analysis (GC-FAME), substrate utilization patterns (BiologTM assays), 16S rRNA sequence comparisons, and total DNA:DNA hybridizations. Microbial respirometry and chemical analyses were also performed to define the PAH-biodegradation potential of these soils. These studies showed that all soils contaminated with PAHs developed competent PAH-degrading bacteria that are biochemically similar and phylogenetically related. However, bioremediation strategies relying exclusively on indigenous PAH degraders should be closely evaluated for the ability to achieve site-specific clean up standards in a timely manner. |
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