Please do not bookmark specific publications. The URL for each item changes each quarter as our Research Products database is refreshed. If you have a question regarding this publication, use the "Contact Us" feature above and include the product citation in your message.

Research Product

Eaton, Richard W. and Sherry S. Wilkinson. Unpublished. Construction of IpbR/XylS Hybrid Regulators: A Role for both HTH-1 and HTH-2 of XylS/AraC Family Regulators in DNA Recognition. Appl. Environ. Microbiol. 17 p. (ERL,GB 1156).

Related (56% identity) positive regulator proteins, IpbR and XylS, control expression of isopropylbenzene and m-toluate catabolic pathways, respectively. To investigate the interchangeability of homologous domains, plasmids carrying wild-type and hybrid ipbR and xylS were constucted and introduced into E. coli cells containing either ipbOP-lacZ or xylOmPm-lacZ. Following induction of these cells with isopropylbenzene or m-toluate, cell extracts were assayed for b-galactosidase. Wild-type regulator proteins gave inducible expression of the corresponding OP-lacZ. A noteworthy hybrid regulator encoded by pSSW12, which carries the first 251 amino acids of XylS (including HTH-1) and the last 81 amino acids of IpbR (including HTH-2), activated expression of both xylOmPm-lacZ and ipbOP-lacZ when induced with m-toluate. This suggests that both helix-turn-helix regions are involved in operator DNA recognition.

horizontal blue bar

[ ORD Home | NHEERL Home ]

Research Product

Local Navigation