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Devereux, Richard and Stephanie G. Willis. 1995. Amplification of Ribosomal RNA Sequences. In: Molecular Microbial Ecology Manual. A.D.L. Akkermans, J.D. van Elsas, and F.J. DeBruin, Editors. Kluwer Academic Publishers, Norwell, MA. Pp. 3.3.1:1-11. (ERL,GB 881).

This book chapter offers an overview of the use of ribosomal RNA sequences. A history of the technology traces the evolution of techniques to measure bacterial phylogenetic relationships and recent advances in obtaining rRNA sequence information. The manual also describes procedures for the isolation of microbial nucleic acids from pure cultures and environmental samples and preparation of DNA templates.

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