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Grizzle, John M., Marshall R. Putnam, John W. Fournie and John A. Couch. 1988. Microinjection of Chemical Carcinogens into Small Fish Embryos: Exocrine Pancreatic Neoplasm in Fundulus grandis Exposed to N-Methyl-N'-Nitro-N-Nitrosoguanidine. EPA/600/J-88/282. Dis. Aquat. Org. 5(2):101-105. (ERL,GB X571). (Avail. from NTIS, Springfield, VA: PB89-208698)
Gulf killifish, Fundulus grandis, embryos were injected with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Retention of carcinogen in the egg immediately after injection was highly variable. Hatching percentages of MNNG-injected and uninjected embryos were similar, but survival of MNNG-injected fish during the first week after hatching was less than half of the survival of controls. One of three MNNG-exposed fish examined after 20 weeks had a pancreatic acinar cell carcinoma, a type of neoplasm seldom found in fish. No neoplasms were found in controls nor in MNNG-exposed fish examined after 34 weeks. The neoplasm was invasive and had a high mitotic index, but no metastases were found. The acinar cell origin of the tumor was confirmed by ultrastructural examination of tissue reprocessed from paraffin into epoxy resin. |
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