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Cuskey, Stephen M. 1990. Use of Lethal Bacterial Genes to Limit Survival of Intentionally Released Genetically Engineered Microorganisms. In: Review of Progress in the Biotechnology-Microbial Pest Control Agent Risk Assessment Program. EPA/600/9-90/029. U.S. Environmental Protection Agency, Environmental Research Laboratory, Corvallis, OR and Environmental Research Laboratory, Gulf Breeze, FL. Pp. 197-199. (ERL,GB X675).

The construction and testing of cells containing conditionally-lethal constructs under a variety of genetic and environmental conditions in the laboratory are reported. Also discussed are preliminary attempts to design and construct conditionally-lethal counter-selection transposons for use in gene transfer experiments and attempts to determine the lethal mechanism of action of kilA which has not been previously reported.

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