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Microbiological and Chemical Exposure Assessment

Giardia Image Sequence 2

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Differential interference contrast (DIC) image of Giardia lamblia

Photo Credit: H.D.A Lindquist, U.S. EPA

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Differential interference contrast (DIC) image of Giardia lamblia cysts, purified from Mongolian gerbil fecal material. Cysts are ovoidal or elipsoidal objects, usually 11-14 microns in length. Cysts may contain as many as 4, nuclei, and residual structures from their trophozoite or vegetative form. These residua include central axonemes, remnants of the striated disk, and remnant median bodies. In some cysts these structures will be indistinct. Scale bar is 10 microns.

Immunofluorescence image of Giardia lamblia

Photo Credit: H.D.A Lindquist, U.S. EPA

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Immunofluorescence image of Giardia lamblia cysts, purified from Mongolian gerbil fecal material. (Same field of view) Cysts were stained with commercially available immunofluorescent antibodies. Cysts should have an intense apple green fluorescence on the periphery of their cyst wall, and measure 11-14 microns in length. Scale bar is 10 microns.

Fluorescence image of Giardia lamblia

Photo Credit: H.D.A Lindquist, U.S. EPA

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Fluorescence image of Giardia lamblia cysts, purified from Mongolian gerbil fecal material. (Same field of view) Cysts were stained with 4,6-diamidino 2-phenyl-indole dihydrochloride (DAPI). DAPI interacts with nucleic acids and stains the nuclei within the cyst. There should be 4 nuclei in each cyst. Cysts that appear to have fewer than 4 stained nuclei, may have 4 nuclei with the others not visible in this plane of focus. Cysts with no nuclei visible, may be dead, may be resistant to DAPI staining, or may be organisms other than G. lamblia.

Photo Credit: These images are in the public domain and are thus free of any copyright restrictions. For public domain images, permission is not required for their use but we do ask that you credit the EPA and the contributors (found in the Photo Credit line) whenever an image is used in any publicly distributed media.

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