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ToxCast™ Data

All available ToxCast Phase II data (including assay summary activity files, assay description files, effect and endpoint data files from animal toxicity studies, concentration response data files & chemical library and structure files) can be downloaded below.

ToxCast Phase II Data & Other Files

Data Set Description Download
Chemical list & Annotation Files (DSS Tox Information) Contains the most current, official public listing of all of the ToxCast 1,800 and Tox21 8,307 chemicals in both an Excel and SD file format. It also contains files used to analyze chemical structure (fingerprinting & SMILEs). More information is provided in the README file available below and at: http://www.epa.gov/ncct/dsstox Download
(10 MB, ZIP)
High-Throughput Chemical Screening Data from ToxCast & Tox21 Contains high-throughput in vitro data on 1,800 chemicals from more than 800 high-throughput screening assays. It also contains concentration response files and curve fits as well as detailed descriptions of all the assays. More information is provided in the README file available below and at: http://www.epa.gov/ncct/toxcast/ and http://actor.epa.gov/actor/faces/CSSDashboardLaunch.jsp Download
(623 MB, ZIP)
Animal Toxicity Studies: Effects & EndPoints (ToxRefDB) File is intended to provide No Effect Level (NEL)/Lowest Effect Level (LEL) and/or No Observed Adverse Effect Level (NOAEL)/Lowest Observed Adverse Effect Level (LOAEL) across the thousands of studies currently in ToxRefDB. More information is provided in the README file available below and at http://actor.epa.gov/toxrefdb/faces/Home.jsp Download
(6.4 MB, ZIP)
Animal Toxicity Studies: Lowest Effect Level Dose for Data Challenges Contains a training set of 500 chemicals for the TopCoder and InnoCentive community to use to help solve the data challenges. The data challenges are asking solvers for ideas on how to use ToxCast data to predict the lowest effect level dose in animals. More information is provided in the README file available below and at http://epa.gov/ncct/challenges.html Download
(24 KB, CSV)
ToxCast Phase II Data Release README File This file is intended to be a brief guide to help navigate and analyze all the ToxCast Phase II data. Download
(481 KB, PDF)

ToxCast Data Use Considerations

  • The activity of a chemical in a specific assay does not necessarily mean that it will cause toxicity or an adverse health outcome. There are many factors that determine whether a chemical will cause a specific adverse health outcome. Careful review is required to determine the use of the data in a particular decision context.
  • Interpretation of ToxCast data is expected to change over time as both the science and analytical methods improve.

ToxCast Data From Published Scientific Manuscripts

Data Set Description Download Publication
Data from measuring hormone mimicry for ToxCast chemicals in T-47D Human Ductal Carcinoma Cells Supplemental data files from Real-Time Growth Kinetics Measuring Hormone Mimicry for ToxCast Chemicals in T-47D Human Ductal Carcinoma Cells published in Chemical Research in Toxicology. Download
(43.4 MB, ZIP)
Rotroff, D., Dix, D., Houck, K., Kavlock, R. J., Knudsen, T. B., Martin, M. T., & Judson, R. (2013). Real-Time Growth Kinetics Measuring Hormone Mimicry for ToxCast Chemicals in T-47D Human Ductal Carcinoma Cells. Chemical Research in Toxicology.
ToxCast Phase II data from 976 ToxCast chemicals across 331 enzymatic and receptor signaling assays. This data is from the NovaScreen ToxCast assay technology Supplemental data files from Profiling 976 ToxCast Chemicals across 331 Enzymatic and Receptor Signaling Assays published in Chemical Research in Toxicology Download
(218.73 MB, ZIP)
Nisha S. Sipes, Matthew T. Martin , Parth Kothiya , David M. Reif , Richard S. Judson , Ann M. Richard , Keith A. Houck , David J. Dix , Robert J. Kavlock, and Thomas B. Knudsen (2013). Profiling 976 ToxCast Chemicals across 331 Enzymatic and Receptor Signaling Assays. Chemical Research in Toxicology.
Data from chemicals screened in ToxCast assays for potential endocrine disruption Data from chemicals screened in ToxCast assays for potential endocrine disruption Download
(0.12 MB, ZIP)
Rotroff et al (2012), Using In Vitro High-throughput Screening Assays to Identify Potential Endocrine Disrupting Chemicals, Environmental Health Perspectives

ToxCast Phase I Data
(Concentration Response Data)

All concentration response data from ToxCast Phase I 
(used to derive AC50/LEC data below)

Download
(22.6 MB, ZIP)

Judson et al. (2010) "In Vitro Screening of Environmental Chemicals for Targeted Testing Prioritization - The ToxCast Project" Environmental Health Perspectives
DOI:10.1289/ehp.0901392

ToxCast Phase 1 Data

Supplemental data and figures for Judson et al

Download
(1.01 MB, ZIP)

Judson et al. (2010) "In Vitro Screening of Environmental Chemicals for Targeted Testing Prioritization - The ToxCast Project" Environmental Health Perspectives
DOI:10.1289/ehp.0901392

ToxCast Phase I Data
(AC50/LEC)

Assay definitions, chemical structures and all summary AC50 and LEC values from ToxCast Phase I

Download (0.72 MB, ZIP)

Judson et al. (2010) "In Vitro Screening of Environmental Chemicals for Targeted Testing Prioritization - The ToxCast Project" Environmental Health Perspectives
DOI:10.1289/ehp.0901392

Xenobiotic Metabolizing Enzyme and Transporter Gene Expression in Primary Cultures of Human Hepatocytes Modulated by ToxCast Chemicals

Supplemental data from this paper

Download
(487 KB, ZIP)

Rotroff et al. (2010) "Xenobiotic Metabolizing Enzyme and Transporter Gene Expression in Primary Cultures of Human Hepatocytes Modulated by ToxCast Chemicals Journal of Toxicology and Environmental Health

Impact of Environmental Chemicals on Key Transcription Regulators and Correlation to Toxicity End Points within EPA's ToxCast Program

Supplemental data from this paper, including the 48 CIS and 25 TRANS assay names and descriptions, the complete concentration response and derived AC50 values, the results of the relative risk permutation test as tables, and figures displaying the results of establishing a global Emax cutoff, the replicate analysis, and the association between PPAR and rat liver tumorigenesis.

Download
(5.67 MB, ZIP)

Martin et al. (2010) "Impact of Environmental Chemicals on Key Transcription Regulators and Correlation to Toxicity End Points within EPA's ToxCast Program"  Chemical Research in Toxicology
DOI: 10.1021/tx900325g

Included in ToxCast Phase 1 data above

Supplemental data from paper including ToxCast chemicals assayed at 4 concentrations in 8 BioMAP cell systems, with a total of 87 assay endpoints resulting in more than 100,000 data points.

See above to download Toxcast Phase 1 data

Houck et al. (2009) "Profiling Bioactivity of the ToxCast Chemical Library Using BioMAP Primary Human Cell Systems" Journal of Biomolecular Screening
DOI: 10.1177/1087057109345525

Evaluation of genotoxicity assays used in profiling ToxCast chemicals

Supplemental data includes complete data table for profiles of all ToxCast chemicals using GreenScreen HC, CellSensor p53 and CellCiphrp53 assays with corresponding Ames and tumorogenicity data.

Download
(49.7 KB, ZIP)

Knight et al. (2009) "Evaluation of high-throughput genotoxicity assays used in profiling the US EPA ToxCast chemicals" Regulatory Toxicology and Pharmacology Journal
DOI:10.1016/j.yrtph.2009.07.004

Landscape Chemicals and Assays

Supplemental data , including the set of chemicals and data sources described in the paper "The Toxicity Data Landscape of Environmental Chemicals". (More Information)

Download (15.5 MB, ZIP)

Judson et al. (2008) "The Toxicity Data Landscape for Environmental Chemicals" Environmental Health Perspectives
DOI:10.1289/ehp.0800168

If a free electronic copy of a research publication is not available on the table below, please contact Monica Linnenbrink (Linnenbrink.Monica@epa.gov)

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