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Superfund Innovative Technology Evaluation (SITE)

 EPA/540/MR-93/527

Note: EPA no longer updates this information, but it may be useful as a reference or resource.

  Augmented In Situ Subsurface Bioremediation ProcessT BIO-REM, Inc.
November 1993

The Augmented In Situ Subsurface Bioremediation ProcessT developed by BIO-REM, Inc., uses microaerophilic bacteria and micronutrients (H-10) and surface tension depressants/penetrants for the treatment of hydrocarbon contaminated soils and groundwater. The bacteria utilize hydrocarbons as electron acceptors and can propagate under very low oxygen tensions thus eliminating the need for additional oxygen sources as well as nutrient treatment. The contaminants are hardy and can thrive over a wide temperature range. Application of the technology is adaptable to site specific situations. The Bio-Rem process was evaluated on JP-4 jet fuel contaminated soils at a former Liquid Fuels Storage Area on Williams Air Force Base (AFB), Phoenix, Arizona, between March 1992 and June 1993. Testing involved the preparation and delivery of 100,000 gallons of H-10 slurry using groundwater from the site to the subsurface soil via a network of over 315 open soil borings. The treatment area was monitored via periodic soil sampling. Samples were analyzed for BTEX, TRPH, and TPH utilizing EPA SW846 methodologies. The developers claimed that organic contaminants in soils between the depths of four to twenty-five feet would be reduced to less than the project specific cleanup levels.

Risk Mangement Research | Air and Climate Change Research | Water Research | Ecosystems Restoration Research | Land Risk Management Research | Technology: Sustainable Technologies Research, Environmental Technology Verification Program (ETV), and Technology Assessments

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