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 Abstract

  Callus Cell Formation and Suspension Culture of Typha latifolia (EPA/600/JA-02/227) 2002

This study describes the first reported attempt to generate a growth curve from Typha latifolia L. (broadleaf cattail) callus cells in suspension culture. Several media and hormone combinations were tested for their capacity to induce callus cell formation from T. latifolia leaf sections and both male and female inflorescence spikes.

A T. latifolia callus cell line was successfully established from immature female inflorescence spikes. Callus growth on Gamborgs B5 medium supplemented with 5 mg/L(-1) dicamba and 1 mg/L(-1) BA was greater than that on other media. A growth curve was generated for callus growth on the most favorable culture medium in suspension culture. The mass of the cells increased by 150 percent by the end of the growth curve.

Researchers have reported that this species can remove pollutants from storm water wetlands very effectively. T. latifolia grows in freshwater marshes, wet swales, streams, and ponds, and along lake margins. It is found in all fifty states and is among the most common aquatic plants. It can dominate large areas, especially where water levels fluctuate. In the early spring, it rapidly forms dense colonies that slow down storm water flows and allow particles to settle into the sediment. It is also known to uptake from storm water wetlands such nutrients and heavy metals as phosphorous, nitrogen, copper, nickel, zinc, and magnesium, which are then stored in all parts of the plant, including the flower. This makes it an excellent candidate for use in storm water wetlands.

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Marie O’Shea


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