EPA/600/R-09/103

The myriad of in situ subsurface remediation technologies currently in practice results in responses by indigenous or introduced microbial communities that can be measured with respect to alterations in biomass, structure, diversity, enzymatic activity, or consequent stress. Providing a highly developed understanding of subsurface ecologies has shown great promise, through the use of molecular and genomic techniques, in providing new approaches to soil and ground water investigations by reducing the inherent parameter variability of more traditional approaches in bench- and pilot-scale investigations, as well as full-scale applications. In addition to providing a background on classic molecular and genomic sciences, the results and interpretation of their application to field-scale subsurface remediation activities is discussed.

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Ann Keeley

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