Skip common site navigation and headers
United States Environmental Protection Agency
Unregulated Contaminant Monitoring Rule 1 (UCMR 1)
Begin Hierarchical Links EPA Home > Water > Ground Water & Drinking Water > UCM Home > UCMR 1 > Regulations > Monitoring for Aeromonas as a UCMR List 2 Contaminant: Voluntary Performance Testing Program for Laboratory Approval End Hierarchical Links

 

Monitoring for Aeromonas as a UCMR List 2 Contaminant: Proficiency Testing Program for Laboratory Approval

A Final Rule was published October 29, 2002, (67 FR 65888) which identified the approved analytical method for Aeromonas monitoring as a UCMR List 2 contaminant and the laboratory approval program. Below is a copied portion of the Rule where this program is specified.

IV. Laboratory Approval and Certification for Aeromonas Monitoring
As a result of today's action, laboratories wishing to analyze samples for Aeromonas under the UCMR must use EPA Method 1605. EPA has previously specified, in Sec. 141.40 (a)(5)(ii)(G)(3) (66 FR 2273, January 11, 2001), that Aeromonas analyses must be performed by laboratories certified under Sec. 141.28 for compliance analyses of coliform indicator bacteria using an EPA approved membrane filtration procedure. Because of differences between EPA Method 1605 and existing membrane filtration methods for coliform indicator bacteria, laboratories performing EPA Method 1605 must also participate in proficiency testing (PT) studies to be conducted by EPA. Laboratories wishing to be approved to use Method 1605 for this monitoring should submit a ``request to participate'' letter to EPA and will be asked to analyze 10 samples for Aeromonas using Method 1605. Within 10 days of this rule being signed by the EPA Administrator, EPA will notify each large public water system selected to perform Aeromonas monitoring of the need for their laboratory to submit this "request to participate" letter. EPA has established 30 days following the publication of the final rule as the latest date by which it will be able to accept the "request to participate" letter due to the very short time left before the beginning of the monitoring program (January 2003). The "request to participate" letter should be mailed to: Technical Support Center Aeromonas PT Coordinator, EPA, 26 West Martin Luther King Drive, Cincinnati, Ohio 45268. Upon completion of the Aeromonas PT Program, EPA will provide each successful laboratory with an approval letter identifying the laboratory by name and the approval date. This letter and a copy of the laboratory's certification under Sec. 141.28 for compliance analysis of coliform indicator bacteria using an EPA approved membrane filtration procedure, may then be presented to any public water system (PWS) as evidence of laboratory approval for Aeromonas analysis supporting the UCMR. Laboratory approval is contingent upon the laboratory having and maintaining certification to perform drinking water compliance monitoring using an approved coliform membrane filtration method. EPA will post a list of the laboratories that have successfully completed each PT study.

A laboratory's request letter should include the following information:

  1. Laboratory Name
  2. Complete Laboratory Mailing Address
  3. Proof of the laboratory's certification under §141.28 for compliance analysis of coliform indicator bacteria using an USEPA approved membrane filtration procedure
  4. A copy of the laboratory's Initial Demonstration of Capability (IDC) as well as blank and negative culture control data as specified in Sections 9.4 through 9.6 of Method 1605. A summary table for this information is attached.
  5. Contact Person at the lab
  6. Contact Phone, FAX, and e-mail (if available)

The "request letter" package should be mailed to:

Technical Support Center Aeromonas PT Program Coordinator
U.S. Environmental Protection Agency (MS-140)
26 W. Martin Luther King Drive
Cincinnati, Ohio 45268

Laboratories should also FAX (513-569-7191) the request letter with certification confirmation. We suggest laboratories request receipt confirmation to ensure registration.

Some important FAQs about the Aeromonas PT Program and monitoring....

How much demand will there be for UCMR List 2 monitoring of Aeromonas?
If your laboratory is interested in participating in this voluntary PT program, they should consider the fact that commercial laboratory demand for the analysis of the List 2, Screening Survey contaminant Aeromonas, will be very limited. Commercial laboratories which gain Aeromonas approval will only be competing to support the monitoring at those 120 large public water systems (PWS) which have been selected as part of the List 2 Microbiological Screening Survey. The complete list of systems required to monitor for List 2, Aeromonas can be found in Table 2.

Be certain to specifically look toward the middle of this page for Table 2, where the List 2, Aeromonas systems are listed. Each of these systems will need to collect a total of 18 samples from defined locations in their distribution system throughout 2003. EPA has laboratory contracts established for the 180 small PWSs which are also required to monitor.

What is the schedule for the PT study and deadline for submitting request letters?
Two voluntary Aeromonas PT studies occured in April and August 2002. The third and final PT study is scheduled for December 2, 2002 with a deadline for request letter submission of November 29, 2002. Laboratories need to pass one of these studies to qualify for approval. Laboratories which failed a voluntary PT study will not automatically be enrolled in the third study and will need to resubmit a request. This will ensure that the laboratory is still interested in pursuing Aeromonas laboratory approval.

How will approval to conduct Aeromonas monitoring be granted?
After the laboratory has submitted its IDC data, provided proof of certification, and successfully passed the PT, it will receive a letter indicating its approval. Laboratory approval will be limited and specific to the duration of the UCMR (1999) Aeromonas monitoring period.

Application for Participation in Aeromonas Proficiency Testing Program

Laboratory Name:
Contact Person:
Complete Mailing Address:


Phone: Fax:
E-mail Address:

Initial Demonstration of Capability Data

 

Aeromonas CFU

Colony Color

Oxidase Result

Trehalose Result

Indole Result

9.4 Initial Precision and Recovery

       

Spiked Reagent Water #1

         

Spiked Reagent Water #2

         

Spiked Reagent Water #3

         

Spiked Reagent Water #4

         

Mean CFU

         

RSD of CFU

         

9.5 Blank

         

9.4.3 Positive Control

A. hydrophila

         

9.6.4.1 Oxidase Negative Control (E. coli)

         

9.6.4.2 Trehalose Negative Control (P. aeruginosa)

         

9.6.4.3 Indole Negative Control (B. cereus)

         
Mail to:
Technical Support Center Aeromonas PT Program Coordinator
U.S. Environmental Protection Agency (MS-140)
26 W. Martin Luther King Drive
Cincinnati, Ohio 45268
Fax to:
513-569-7191

Include a copy of proof of certification for coliforms by membrane filtration.

 

Safewater Home | About Our Office | Publications | Calendar | Links | Office of Water | En Español

 
Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us