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Bacillus subtilis var. amyloliquefaciens strain FZB24 (006480) Biopesticide Registration Action Document

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  1. Abstract
  2. Overview
    1. Product Overview
    2. Use Profile
    3. Regulatory History
  3. Science Assessment
    1. Physical and Chemical Properties Assessment
    2. Human Risk Assessment
    3. Environmental Assessment
  4. Risk Management and Re/Registration Decision
    1. Determination of Eligibility
    2. Regulatory Position
    3. Labeling Rational
  5. Actions Required By Registrants
    1. Precautionary Labeling
    2. Environmental Hazards Labeling
    3. Labeling
    Biblography

  1. Abstract

    2. See  Fact Sheet

  2. Overview

    1. Overview

      • Microbial Pesticide Name: Bacillus subtilis var. amyloliquefaciens strain FZB24


      • Trade Name(s): TAE-022 WDG


      • OPP Chemical Code: 006480


      • Basic Manufacturer: Taensa Inc.


      • US Agent:
        Edgar R. Butts, Ph.D.
        26 Sherman Ct.
        P.O. Box 764
        Fairfield, Ct 06430

    2. Use Profile

      3. Type of Pesticide: Microbial Fungicide and Plant Regulator

      Mode of action: Bacillus subtilis var. amyloliquefaciens strain FZB24 is a naturally occurring soil microorganism. The mode of action appears to be an enzyme production, which acts as an antifungal agent and plant growth promoter.

      Food Clearances/Tolerances: There are no food uses associated with this action, therefore, a tolerance establishment/exemption is not an issue for the proposed uses.

      Use Sites: At this time, Bacillus subtilis var. amyloliquefaciens strain FZB24 is only registered for greenhouse and indoor use.

      Greenhouse Food: None

      Greenhouse and Indoor Non-Food: Ornamentals, shrubs, shade and forest tree seedlings.

      Target Pests for Active Ingredient: TAE-022 is used for plant strengthening, enhancing, growth, increasing yields, and for suppressing selected soil-born diseases such as Rhizoctonia and Fusarium.

      Formulation Types Registered:

      Form

      Technical - 73.3% (5.3 H 1011 cfu/g) Bacillus subtilis var. amyloliquefaciens strain FZB24.

      End-use product - water dispersible granule; 24.5% (5.0 H 1010 cfu/g) Bacillus subtilis var. amyloliquefaciens strain FZB24

      Method and Rates of Application:

      Types of Treatment:  Water dispersible granules should be pre-mixed thoroughly with water to assure a properly concentrated suspension. For best results, apply TAE-022 to seedlings or to newly rooted cuttings.

      Equipment: None specified

      Timing: Apply content of entire suspension within a few hours of mixing to ensure viability of TAE-022. Apply TAE-022 as early as possible in the life cycle of the plant to enhance growth and disease resistance. TAE-022 should be applied to plants every few weeks for up to three to four applications as needed.

      Rates of Application:

      Drenching: Per 100 gallons of water - by weight use 75 grams or 2.6 ounces; by volume use 3.5 fluid ounces. Per one gallon of water - by weight use 0.75 grams; by volume use 0.2 teaspoon.

      Cutting and Root Dips: Per 10 gallons of water - by weight use 40 grams; by volume use 1.8 fluid ounces. Per one gallon of water - by weight use 4 grams; by volume use one teaspoon. Per one liter of water - by weight use one gram.

      Transplants, Including Plugs: Dip or Drench until root system is thoroughly soaked

      Method of Application: When drenching, apply TAE-022 to seedlings or to newly rooted cuttings. Cutting and root dips should be placed in the suspension for five to ten minutes allowing time for TAE-022 to penetrate the rootzone. Ornamentals should receive at least one follow-up drench treatment two to three weeks following initial treatment. Transplants and plugs root system should be dipped or drenched prior to planting.

    3. Regulatory History

      4. Taensa Inc. submitted an application February 8, 1999 for registration of TAE-022 Technical and TAE-022 WDG; active ingredient Bacillus subtilis var. amyloliquefaciens strain FZB24.

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  3. Science Assessment

    1. Physical and Chemical Properties Assessment

      2. Product Identity:

      The agency has classified Bacillus subtilis var. amyloliquefaciens strain FZB24 as a microbial pesticide because it is a naturally occurring soil microorganism. Bacillus subtilis var. amyloliquefaciens strain FZB24 was shown to grow from 30EC to 50EC. Bacillus subtilis var. amyloliquefaciens strain FZB24 is recognized by Deutsche Sammlung von Mikroorganism (DSM): DSM ID 96-2, with an optimal antifungal activity in minimal media (e.g., SNA media) at cooler temperatures (e.g., 17EC), and on a broad spectrum scale.

      Product chemistry data which support the registration of Bacillus subtilis var. amyloliquefaciens strain FZB24 are summarized in Table 1.

      Table 1. Physical and Chemical Properties for Bacillus subtilis var. amyloliquefaciens strain FZB24.
      Guideline No Study Results MRID No.
      OPPTS 885.1100 Product Identity and Disclosure of Ingredients Acceptable 447581-01
      OPPTS 885.1200 Manufacturing Process Acceptable 447581-02
      OPPTS 885.1300 Formation of Unintentional Ingredients Acceptable 447581-03
      OPPTS 885.1400 Analysis of Samples Acceptable 447581-04
      OPPTS 885.1500 Certification of Limits Limits listed in the CSF are adequate 447581-05
      OPPTS
      830.6302,
      830.6303,
      830.6304,
      830.7000,
      885.7300      		 Product Chemistry Acceptable. TAE-022 Technical is beige, solid, acidic odor, a bulk density of 0.186 g/mL, and pH 6.57 (20EC). TAE-022 WDG is beige, solid, carbonyl odor, bulk density of 0.739 g/mL, and pH 6.52 (20EC) 447581-06
      447581-07
      OPPTS 885.1100 Growth Parameters at Various Temperatures Acceptable. Bacillus subtilis var. amyloliquefaciens strain FZB24 grows from 4.2 H 1011 cfu/g at 37EC to 6.0 H 1011 cfu/g at 34EC. 447581-19
      OPPTS 885.1100 Sensitivity Detection Acceptable. The detection method for Bacillus subtilis var. amyloliquefaciens strain FZB24 was demonstrated. 447581-20

    2. Human Risk Assessment

      3. The Bacillus subtilis var. amyloliquefaciens strain FZB24 acute oral, pulmonary, and intravenous toxicity tests were conducted according to Agency guidelines and demonstrated little to no potential adverse effects. There is, therefore, a reasonable certainty that no harm will result from exposure to Bacillus subtilis var. amyloliquefaciens strain FZB24. This includes all anticipated dietary exposures and all other exposures for which there is reliable information.

      Although B. subtilis is not a frank human pathogens, there have been strains isolated from human infections. However, such occurrences are typically limited to patients who are immuno-compromised because of some other condition or disease. In addition, the organism does produce the enzyme subtilisin which has been reported to produce allergenic or hypersensitivity reactions to individuals repeatedly exposed to the enzyme. However, OSHA has established standards for industrial settings (the only likely potential for repeated exposure) for exposure limits to workers.

      Overall, B. subtilis produces only a very small potential risk to human health. The organism is not a frank pathogen, but is similar to numerous other opportunistic pathogens which are ubiquitous in nature. In all likelihood, individuals who may be sensitive to the organism have already been exposed to it, and risk of increased exposure is likely only to exist for pesticide applicators and manufacturers of the product. These individuals should be equipped with adequate personal protection equipment.

      1. Human Toxicity Assessment


        1. Acute Toxicity
          2.

          All mammalian toxicology data requirements have been submitted and adequately satisfy data requirements to support registration. The acute oral, acute pulmonary toxicity/pathogenicity, and acute eye irritation studies resulted in Toxicity Category III classification and acute dermal irritation studies resulted in Toxicity Category III for the end-use product. The technical or manufacturing product has a Toxicity Category I for eye irritation, Toxicity Category III for dermal irritation, and Toxicity Category III for other exposures.

          Table 2. Toxicity Data Requirements
          Guideline No Study Results MRID No.
          OPPTS 885.3050 Acute Oral Toxicity/ Pathogenicity - Technical Acceptable. Not toxic/pathogenic to rats dosed at 1.3 H 108 cfu.   447581-08
          OPPTS 870.1100 Acute Oral Toxicity - End-Use Product Acceptable, Toxicity Category III. Toxic/limit dose in rats is >2.8 g/kg body weight (6.7 H 1010 cfu/kg). 447581-09
          OPPTS 870.2500/885.3100 Acute Dermal Toxicity/Pathogenicity - Technical Acceptable, Toxicity Category III. The LD50 in rats is >2000 mg/kg. 447581-10
          OPPTS 870.2500/885.3100 Acute Dermal Toxicity/Pathogenicity - End-Use Product Acceptable, Toxicity Category III. The LD50 in rats is >2000 mg/kg. 447581-11
          OPPTS 870.2500/885.3100 Acute Dermal Irritation - Technical Acceptable, Toxicity Category II. 447581-10
          OPPTS 870.2500/885.3100 Acute Dermal Irritation - End-Use Product Acceptable, Toxicity Category II. 447581-11
          OPPTS 885.3150 Acute Pulmonary Toxicity/ Pathogenicity - Technical Acceptable. Not toxic/pathogenic to rats dosed at 1.3 H 108 cfu. 447581-12
          OPPTS 885.3200 Acute Intravenous Toxicity/Pathogenicity - Technical Acceptable. Not toxic/pathogenic to rats dosed at 1.7 H 108 cfu. 447581-13
          OPPTS 870.2400 Primary Eye Irritation - Technical Acceptable, Toxicity Category I. 447581-14
          OPPTS 870.2400 Primary Eye Irritation - End-Use Product Acceptable, Toxicity Category III 447581-15
          OPPTS 885.3400 Hypersensitivity Incidents - Human Exposure Acceptable. No hypersensitivity incidents reported. If any are found, they must be reported. 447581-16
          OPPTS 870.2600 Hypersensitivity Testing - Technical Acceptable. Not a sensitizer when dosed at 3.6 H 1010 cfu. 447581-17
          OPPTS 885.1100 Potential Health Effects - Review of Literature Acceptable. No apparent negative effects from Bacillus subtilis var. amyloliquefaciens strain FZB24 447581-18

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        2. Subchronic Toxicity and Chronic Toxicity
          3.

          Subchronic and chronic toxicity were not required because survival, replication, infectivity, toxicity, or persistence of the microbial agent was not observed in the test animals treated in the infectivity tests for oral, pulmonary and injection exposures.

      2. Effects on the Immune and Endocrine Systems
        3.

        The Agency is not requiring information on the endocrine effects of this biological pesticide at this time. The Agency has no information to suggest that Bacillus subtilis var. amyloliquefaciens strain FZB24 has an effect on the immune and endocrine systems. No specific tests have been conducted with Bacillus subtilis var. amyloliquefaciens strain FZB24 to determine such effects. However, as is expected from a non-pathogenic microorganism, the submitted toxicity/pathogenicity studies in rodents indicated that following several routes of exposure, the immune system is still intact and able to process and clear the active ingredient. There are no reports indicating that Bacillus subtilis var. amyloliquefaciens strain FZB24 produces any toxins or antibiotics. Therefore, it is unlikely that this organism would have estrogenic or endocrine effects because it is practically non-toxic to mammals.

      3. Dose Response Assessment
        4.

        No toxicological endpoints are identified.

      4. Dietary Exposure and Risk Characterization
        5.

        The use of Bacillus subtilis var. amyloliquefaciens strain FZB24 is not expected to result in any new dietary exposure to this organism. Bacteria such as Bacillus subtilis var. amyloliquefaciens strain FZB24 are ubiquitous in the agricultural environment. It is anticipated that the concentrations of Bacillus subtilis var. amyloliquefaciens strain FZB24 on treated plants may be elevated immediately after application but will rapidly decline to environmental background levels. The risks anticipated for dietary exposure are considered low because Bacillus subtilis var. amyloliquefaciens strain FZB24 was not toxic/pathogenic to rats dosed at 1.3 H 108 cfu. . (Toxicity Category III)

      5. Occupational, Residential, School and Day care Exposure and Risk Characterization
        6.


        1. Occupational Exposure and Risk Characterization
          2.

          Dermal and inhalation exposure would be the primary routes of exposure for mixer/loader applicators. Since unbroken skin is a natural barrier to microbial invasion of the human body, dermal absorption could occur only if the skin were cut, if the microbe were a pathogen equipped with mechanisms for entry through or infection of the skin, or if metabolites were produced that could be dermally absorbed. Bacillus subtilis is not considered to be a human pathogen nor is it known to produce metabolites that are dermally absorbed. Since the intravenous study demonstrated no adverse effects, it is the Agency's opinion that even cut skin should not pose a risk to health if Bacillus subtilis var. amyloliquefaciens strain FZB24 was absorbed into the body via entry through a cut. The dermal irritation study demonstrated a potential for skin irritation (Toxicity Category II). The acute pulmonary toxicity/pathogenicity study demonstrated that Bacillus subtilis var. amyloliquefaciens strain FZB24 is not toxic/pathogenic to rats dosed at 1.3 H 108 cfu. Adverse effects from inhalation exposure are, therefore, considered minimal. The required personal protective equipment for applicators and handlers is expected to adequately reduce dermal or inhalation exposure to this microbial pesticide.

          Occupational exposures and subsequent risks are negligible because the organism has been determined not to be pathogenic to humans and animals. The risks are expected to be minimal based on evaluations of submitted Tier I acute toxicity tests (Table 2).

        2. Residential, School and Day Care Exposure and Risk Characterization
          3.

          Bacillus subtilis var. amyloliquefaciens strain FZB24 is currently limited to greenhouse and indoor use. The potential risks to humans, including children, are considered negligible based on the lack of significant toxicological concerns, as demonstrated in the Agency's evaluation of the mammalian toxicology studies, low application rates, and low exposure subsequent to application due to volatilization.

          The primary toxicological concerns for humans are dermal irritation and/or sensitization from dermal exposure at high concentrations. The potential of significant exposure to children from dermal exposure is anticipated to be considerably less than that used in exposing experimental animals in tests. Therefore, additional requirements for evaluation of exposure over that mitigated by the precautionary labeling statements currently proposed (refer to Section V) are not necessary. In addition, systemic toxicity is not expected to occur from pesticide uses since dermal irritation occurs at high doses and typically results in self-discontinuation of product use.

      6. Drinking Water Exposure and Risk Characterization
        7.

        Bacillus subtilis var. amyloliquefaciens strain FZB24 is a naturally-occurring microorganism. Bacillus subtilis is widespread in the environment throughout the world. Although Bacillus subtilis spores may be found in water, it is not known as an aquatic bacterium, and therefore is not expected to proliferate in aquatic habitats. Moreover, the risk from non-occupational exposure is considered minimal as there is no evidence of adverse effects from oral, dermal, or inhalation exposure to this microbial agent is not considered to be a risk to drinking water. Drinking water is accordingly not being screened for Bacillus subtilis as a potential indicator of microbial contamination or as a direct pathogenic contaminant. Both percolation through soil and municipal treatment of drinking water would reduce the possibility of exposure to Bacillus subtilis through drinking water. Therefore, the potential of significant transfer to drinking water is minimal to nonexistent. In addition, the risk from consumption of drinking water containing Bacillus subtilis is considered minimal as there is no evidence of adverse effects from oral exposure to this microbial agent.

      7. Acute and Chronic Dietary Risks for Sensitive Subpopulations Particularly Infants and Children
        8.

        Although there have been reports of B. subtilis causing allergic reactions, particularly when added to laundry detergent, no confirmed reports of immediate or delayed allergic reactions to Bacillus subtilis var. amyloliquefaciens strain FZB24 have been reported.

        Based on the acute toxicity information discussed above, EPA concludes that there is a reasonable certainty that no harm will result from aggregate exposure to the United States population, including infants and children, to residues of Bacillus subtilis var. amyloliquefaciens strain FZB24. This includes all anticipated dietary exposures and all other exposures for which there is reliable information. The Agency has arrived at this conclusion because, as discussed in Unit B. Human Risk Assessment, Bacillus subtilis var. amyloliquefaciens strain FZB24 is practically non-toxic to mammals and under reasonably foreseeable circumstances it does not pose a risk.

      8. Aggregate Exposure from Multiple Routes Including Dermal, Oral, and Inhalation
        9.

        Bacillus subtilis var. amyloliquefaciens strain FZB24 is currently limited to greenhouse and indoor use. The potential risks to humans, including children, are considered negligible based on the lack of significant toxicological concerns, as demonstrated in the Agency's evaluation of the mammalian toxicology studies, low application rates, and low exposure subsequent to application due to volatilization.

        The primary toxicological concerns for humans are dermal irritation and/or sensitization from dermal exposure at high concentrations. The potential of significant exposure to children from dermal exposure is anticipated to be considerably less than that used in exposing experimental animals in tests. Therefore, additional requirements for evaluation of exposure over that mitigated by the precautionary labeling statements currently proposed (refer to Section V) are not necessary. In addition, systemic toxicity is not expected to occur from pesticide uses since dermal irritation occurs at high doses and typically results in self-discontinuation of product use.

      9. Cumulative Effects
        10.

        Bacillus subtilis var. amyloliquefaciens strain FZB24 is practically non-toxic to mammals. No mechanism of toxicity in mammals has been identified for this organism. Therefore, no cumulative effect with other toxins or related organisms is anticipated.

    3. Environmental Assessment


      1. Environmental Fate
        2.

        Environmental fate data were waived because Bacillus subtilis var. amyloliquefaciens strain FZB24 is a naturally occurring microorganism found in soil and plant litter. The ubiquitous nature of the organism suggests that the environmental burden is not likely to increase substantially with the proposed limited indoor and greenhouse use patterns of the microbial pesticide.

      2. Ecological Toxicity
        3.

        The intended applications of Bacillus subtilis var. amyloliquefaciens strain FZB24 to greenhouse non-food crops including ornamentals, shrubs, shade and forest tree seedlings, and indoor use patterns are generally not expected to pose adverse risks to non-target organisms. Phytotoxicity to grapes and rot to potato tubers may be possible and further testing or information is necessary to quantify these risks. Non-target organism testing data are not required for these use patterns of Bacillus subtilis var. amyloliquefaciens strain FZB24.

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  4. Risk Management and Re/Registration Decision

    1. Determination of Eligibility

      2. Section 3(c)(5) of FIFRA provides for the registration of new active ingredients if it is determined that

      1. its composition is such as to warrant the proposed claims for it;
      2. its labeling and other materials required to be submitted comply with the requirements of FIFRA;
      3. it will perform its intended function without unreasonable adverse effects on the environment; and
      4. when used in accordance with widespread and commonly recognized practice, it will not generally cause unreasonable adverse effects on the environment.

      To satisfy criterion "A" above, Bacillus subtilis var. amyloliquefaciens strain FZB24 has well known properties. The Agency has no knowledge that would contradict the claims made on the label of this product. Criterion "B" is satisfied by the current label and by the data presented in this document. It is believed that this new pesticidal active ingredient will not cause any unreasonable adverse effects, is a broad spectrum microbial fungicide, and does provide protection as claimed satisfying criterion "C". Criterion "D" is satisfied in that Bacillus subtilis var. amyloliquefaciens strain FZB24 is not expected to cause unreasonable adverse effects when used according to label instructions.

      Therefore, Bacillus subtilis var. amyloliquefaciens strain FZB24 is eligible for registration. The uses are listed in the Section II, B. Use Profile. These eligible uses are limited to indoor, greenhouse food and greenhouse-non -food sites. There are no ineligible uses for Bacillus subtilis var. amyloliquefaciens strain FZB24.

    2. Regulatory Position

      1. Unconditional Registration: The data requirements are fulfilled and the Biopesticides and Pollution Prevention Division recommends unconditional registration of products that contain Bacillus subtilis var. amyloliquefaciens strain FZB24 as the sole Active ingredient (TAE-022 Technical and TAE-022 WDG).


      2. Tolerances for Food Uses and /or exemptions: Bacillus subtilisvar. amyloliquefaciens strain FZB24 is not registered food uses. A tolerance was, therefore, not required.


      3. CODEX Harmonization: There are no CODEX values for Bacillus subtilis var. amyloliquefaciens strain FZB24.


      4. Risk Mitigation: Since there are no risk issues, no risk mitigation measures are required at this time for dietary risk, occupational and residential risk, or ground and surface water contamination for Bacillus subtilis var. amyloliquefaciens strain FZB24.


      5. Endangered Species Statement: Based on the use pattern and the results of toxicity and exposure studies, the Agency has determined that this action will have no effect on listed species.

    3. Labeling Rational

      1. Human Health Hazard (WPS and non-WPS)
        2.

        Bacillus subtilis var. amyloliquefaciens strain FZB24 products with commercial use sites are subject to the Worker Protection Standard. Due to the low toxicity of Bacillus subtilis var. amyloliquefaciens strain FZB24, the Re-Entry Interval for uses within the scope of WPS is four hours. Precautionary statements and personal protective equipment as specified below are required based on the acute toxicity categories of this organism.

      2. Environmental Hazard
        3.

        Precautionary labeling is required as indicated below.

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  5. Actions Required By Registrants

    1. Precautionary Labeling

      2. Bacillus subtilis var. amyloliquefaciens strain FZB24 products must state the following under the heading "Precautionary Statements":

      Personal Protective Equipment

      Applicators and other handlers must wear: Long sleeved shirt and long pants. Waterproof gloves. Shoes plus socks. Dust/mist filtering respirator (MSHA/NIOSH approval number prefix TC-21C), or a NIOSH approved respirator with any N, P, R, or HE filter.

      Follow manufacturers instructions for cleaning/maintaining PPE. If no such instructions for washables, use detergent and hot water. Keep and wash PPE separately from other laundry.

      WPS labels must state the following under the heading "User Safety Recommendations"

      Users should wash hands before eating, drinking, chewing gum, using tobacco, or using the toilet.

      Users should remove clothing immediately if pesticide gets inside. Then wash thoroughly and put on clean clothing.

      Users should remove PPE immediately after handling this product. If gloves are worn, wash the outside of gloves before removing. As soon as possible, wash thoroughly and change into clean clothing.

    2. Environmental Hazards Labeling

      3. Provided the following statement is placed into the environmental hazards statement, the risk of Bacillus subtilis var. amyloliquefaciens strain FZB24 is minimal to nonexistent to non-target organisms including endangered species.

      1. End-Use Product Environmental Hazards Labeling
        2.

        Do not apply directly to water, or to areas where surface water is present or to intertidal areas below the mean high water mark. Do not contaminate water by cleaning of equipment or disposal of equipment washwaters.

      2. Manufacturing-Use Product Environmental Hazards Labeling
        3.

        Do not discharge effluent containing this product into lakes, streams, ponds, estuaries, oceans, or public water unless this product is specifically identified and addressed in an NPDES permit. Do not discharge effluent containing this product to sewer systems without previously notifying the sewage treatment plant authority. For guidance, contact your State Water Board or Regional Office of the EPA.

      3. Application Rate
        4.

        It is the Agency's position that the labeling for the pesticide products containing Bacillus subtilis var. amyloliquefaciens strain FZB24 as the active ingredient complies with the current pesticide labeling requirements. The Agency has not required a maximum number of applications per a season of this active ingredient.

    3. Labeling
      4.

      The attached label for TAE-022 WDG (EPA file number 72098-L) conforms with the labeling requirements for Bacillus subtilis var. amyloliquefaciens strain FZB24 . Some of the essential label requirements are highlighted below.

      Signal word is "Warning," based on toxicity category II for dermal irritation. The product shall contain the following information:

      • Product Name
      • Ingredient Statement
      • Registration Number
      • "Keep Out of Reach of Children"
      • Signal Word (Warning)
      • Personal Protective Equipment (PPE) Requirements
      • Environmental Hazard Statement
      • Storage and Disposal Statement
      • Agricultural Use Requirements
      • Non-Agricultural Use Requirements
      • Directions for Use

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Biblography

Citations Considered to be part of the Data Base Supporting the Registration of Bacillus subtilis var. amyloliquefaciens strain FZB24

MIRD Citation
44758101 Product Identity and Disclosure of Ingredients
44758102 Beginning Materials and Manufacturing Process
44758103 Discussion of Formation of Unintentional Ingredients
44758104 Analysis of Samples
44758105 Certification of Limits
44758106 Physical/Chemical Properties B Technical
44758107 Physical/Chemical Properties B "WDG" End-Use Product
44758108 Acute Oral Toxicity/Pathogenicity B Technical
44758109 Acute Oral Toxicity B "WDG" End-Use Product
44758110 Acute Dermal Toxicity/Pathogenicity B Technical
44758111 Acute Dermal Toxicity/Pathogenicity B "WDG" End-Use Product
44758112 Acute Pulmonary Toxicity/Pathogenicity B Technical
44758113 Acute Intravenous Toxicity/Pathogenicity B Technical
44758114 Primary Eye Irritation B Technical
44758115 Primary Eye Irritation B "WDG" End Use Product
44758116 Hypersensitivity Incident Reporting
44758117 Hypersensitivity Testing B Technical
44758118 Potential Health Effects of Bacillus subtilis var. amyloliquefaciens strain FZB24 B Review of the Literature
44758119 Growth Parameters of Bacillus subtilis var. amyloliquefaciens strain FZB24 at Various Temperatures
44758120 Sensitivity Detection

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