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Microbiology Laboratory Antimicrobial Testing Methods & Procedures

Methods | Procedures

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Methods

Method ID Number
Title
MB-01-06 (28 pp, 162 Kb) Biosafety in the Laboratory
MB-02-06 (7 pp, 88 Kb) Tracking of Test Microorganisms
MB-03-06 (10 pp, 121 Kb) Screening of Polished Stainless Steel Cylinders, Porcelain Cylinders and Glass Slide Carriers Used in Disinfectant Efficacy Testing
MB-05-13 (22 pp, 274 Kb ) AOAC Use Dilution Method for Testing Disinfectants
MB-06-07 (21pp, 574 Kb) Germicidal Spray Products as Disinfectants (GSPT): Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
MB-07-06 (17 pp, 129 Kb) Tuberculocidal Activity of Disinfectants: II. Confirmative in vitro Test for Determining Tuberculocidal Activityy
MB-09-05 (18 pp, 362 Kb) Disinfectant Towelette Test Against Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
MB-10-04 (16 pp, 155 Kb) Media and Reagents Preparation and Quality Evaluation
MB-11-04 (13 pp, 76 Kb) Neutralization Confirmation Assay for Disinfectant Products Tested against Mycobacterium bovis (BCG)
MB-13-03 (15 pp, 87 Kb) Handling Spills of Biohazardous Material
MB-15-02 (42 pp, 344 Kb) AOAC Sporicidal Activity of Disinfectants Test (Bacillus × porcelain component only)
MB-16-01 (31 pp, 457 Kb) Quantitative Suspension Test Method for Determining Tuberculocidal Efficacy of Disinfectants Against Mycobacterium bovis (BCG) AOAC Use Dilution Method and the AOAC Germicidal Spray Products as Disinfectants Test (Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica)
MB-17-02 (23 pp, 294 Kb) Neutralization Confirmation Procedure for Products Evaluated with the AOAC Use Dilution Method and the AOAC Germicidal Spray Products as Disinfectants Test (Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
MB-19-02 (15 pp, 314 Kb) Standard Operating Procedure for Growing a Pseudomonas aeruginosa Biofilm using the CDC Biofilm Reactor
MB-20-01 (13 pp,169k) Single Tube Method for Measuring Disinfectant Efficacy Against Biofilm Grown in the CDC Biofilm Reactor
MB-21-02 (17 pp, 216 Kb) Quantitative Three Step Method for Measuring the Efficacy of Liquid Sporicides against Spores of Bacillus subtilis on Hard Non-porous and Porous Surfaces
MB-22-03 (8 pp, 102 Kb) Disinfectant Product Preparation and Sampling Procedures
MB-23-02 (17 pp, 123 Kb) Disinfectant Towelette Test: Testing of Mycobacterium bovis (BCG)
MB-24-02 (18 pp, 255 Kb) Testing of Mycobacterium bovis (BCG) Using the Germicidal Spray Products as Disinfectants Test
MB-25-02 (21 pp, 294 Kb) OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces
MB-26-00 (11 pp, 251 Kb) Neutralization of Microbicidal Activity using the OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces
MB-27-01 (20 pp, 298 Kb) Germicidal and Detergent Sanitizing Action of Disinfectants Test
MB-28-04 (14 pp, 844Kb) Production of Spores of Clostridium difficile for Use in the Efficacy Evaluation of Antimicrobial Agents
MB-30-00 (9 pp, 175 Kb) Preparation of hard water and other diluents for antimicrobial products
MB-31-03 (13 pp, 545 Kb) Quantitative Disk Carrier Test Method (QCT-2) Modified for Testing Antimicrobial Products Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard, Non-porous Surfaces
MB-32-00 (24 pp, 506 Kb) Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard Non-porous Surfaces
MB-33-00 (28 pp, 690 Kb) Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Vegetative Bacteria on Inanimate, Hard, Non-porous Surfaces.

Procedures

The following Standard Operating Procedures are pertinent to specific equipment and practices used in the OPP Microbiology Laboratory. The SOPs describe the laboratory's standard practices for calibrating equipment (EQ), performing quality control procedures (QC), preparing reports (ADM), maintaining chain of custody of samples (COC), and assuring quality management practices (QA and QMP).

To request copies of SOPs, send an e-mail to Luisa Samalot. Include the SOP ID number and title. Methods will be sent by e-mail or shipped via US Postal Service, regular mail.

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Procedure ID Number
Standard Operating Procedure
ADM-01-05 (8 pp, 260k) Preparation and Review of Product Performance Reports
ADM-02-05 (10 pp, 98 Kb) Preparation and Review of Standard Operating Procedures (SOPs)
ADM-03-04 (14 pp, 100 Kb) Records and Archives
ADM-04-03 (14 pp, 114 Kb) OPP Microbiology Laboratory Training
ADM-05-03 (8 pp, 198k) Use and Maintenance of Laboratory Notebooks and Project Binders
ADM-06-02 (6 pp, 122 Kb) Customer Feedback
COC-01-06 (18 pp, 190 Kb) Disinfectant Sample Login, Tracking, and Disposal
EQ-01-06 (14 pp, 109 Kb) Calibration and Maintenance of pH Meters
EQ-02-06 (11 pp, 108 Kb) Maintenance and Calibration of Thermometers and Thermometer/Hygrometers
EQ-03-07 (7pp, 214 Kb) Calibration and Maintenance of Weigh Balances
EQ-04-05 (10 pp, 179 Kb) Use and Maintenance of the Beckman DU 520 Spectrophotometer
EQ-05-06 (5 pp, 76 Kb) Calibration and Maintenance of Timers
EQ-06-05 (10 pp, 119 Kb) Calibration of Kimble Class A Burets
EQ-08-06 (6 pp, 229 Kb) Verification of Volume Dispensed and Maintenance of Oxford Automatic Dispensor
EQ-09-02 (12 pp, 199 Kb) Use of Petrifilm and PetriScanŽ
EQ-10-01 (9 pp, 85 Kb) Use and Maintenance of Orbital Shakers
EQ-11-00 (9 pp, 85 Kb) Use and Maintenance of Biological Safety Cabinets
QA-01-04 (11 pp, 78 Kb) Quality Assurance Unit and its Functions
QA-04-05 (8 pp, 133 Kb) Preparation and Maintenance of the Master Schedule
QC-01-05 (18 pp, 145 Kb) Quality Assurance of Purified Water
QC-02-05 (7 pp, 281 Kb) Monitoring of Laboratories for Airborne Contaminants
QC-03-07 (11pp, 204 Kb) Glass Washing and Detergent Residues Test
QC-05-07 (15 pp, 588 Kb) Monitoring Environmental Parameters of Incubators, Refrigerators, Freezers, Laboratories and Sample Storage Rooms
QC-06-05 (11 pp, 104 Kb) Use and Maintenance of Biological Safety Cabinets
QC-09-06 (12 pp, 226 Kb) Establishment of Control Numbers and Tracking of Laboratory Supplies
QC-13-05 (13 pp, 147 Kb) Performance Verification of Autoclaves
QC-18-05 (12 pp, 98 Kb) Sterility Assessment of Disinfectant Product Samples
QC-19-06 (14 pp, 202 Kb) Verification of Digital Pipettes
QC-21-02 (20 pp, 180 Kb) Determining the Presence of Microbial Contamination in Disinfectant Products
QC-22-03 (12 pp, 207 Kb) VITEK 2 Compact:  Use, Maintenance and Quality Control Procedures
QC-23-01 (11 pp, 190 Kb) Performance Verification of the Yamato Drying Oven (Mechanical Convection Oven)

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