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Microbiology Laboratory Antimicrobial Testing Methods & Procedures

Methods | Procedures

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Methods

Method ID Number
Title
MB-01-06 (28 pp, 161.79 Kb) Biosafety in the Laboratory
MB-02-06 (7 pp, 88 Kb) Tracking of Test Microorganisms
MB-03-06 (10 pp, 121 Kb) Screening of Polished Stainless Steel Cylinders, Porcelain Cylinders and Glass Slide Carriers Used in Disinfectant Efficacy Testing
MB-05-13 (22 pp, 273.31 Kb ) AOAC Use Dilution Method for Testing Disinfectants
MB-06-07 (21pp, 574 Kb) Germicidal Spray Products as Disinfectants (GSPT): Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
MB-07-06 (17 pp, 128.29 Kb) Tuberculocidal Activity of Disinfectants: II. Confirmative in vitro Test for Determining Tuberculocidal Activityy
MB-09-05 (18 pp, 361.88 Kb) Disinfectant Towelette Test Against Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
MB-10-04 (16 pp, 154.71 Kb) Media and Reagents Preparation and Quality Evaluation
MB-11-04 (13 pp, 76 Kb) Neutralization Confirmation Assay for Disinfectant Products Tested against Mycobacterium bovis (BCG)
MB-13-03 (15 pp, 86.39 Kb) Handling Spills of Biohazardous Material
MB-15-02 (42 pp, 343.95 Kb) AOAC Sporicidal Activity of Disinfectants Test (Bacillus × porcelain component only)
MB-16-01 (31 pp, 456.27 Kb) Quantitative Suspension Test Method for Determining Tuberculocidal Efficacy of Disinfectants Against Mycobacterium bovis (BCG) AOAC Use Dilution Method and the AOAC Germicidal Spray Products as Disinfectants Test (Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica)
MB-17-02 (23 pp, 293.71 Kb) Neutralization Confirmation Procedure for Products Evaluated with the AOAC Use Dilution Method and the AOAC Germicidal Spray Products as Disinfectants Test (Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
MB-19-02 (15 pp, 314 Kb) Standard Operating Procedure for Growing a Pseudomonas aeruginosa Biofilm using the CDC Biofilm Reactor
MB-20-01 (13 pp,169k) Single Tube Method for Measuring Disinfectant Efficacy Against Biofilm Grown in the CDC Biofilm Reactor
MB-21-02 (17 pp, 216 Kb) Quantitative Three Step Method for Measuring the Efficacy of Liquid Sporicides against Spores of Bacillus subtilis on Hard Non-porous and Porous Surfaces
MB-22-03 (8 pp, 102 Kb) Disinfectant Product Preparation and Sampling Procedures
MB-23-02 (17 pp, 122.06 Kb) Disinfectant Towelette Test: Testing of Mycobacterium bovis (BCG)
MB-24-02 (18 pp, 254.49 Kb) Testing of Mycobacterium bovis (BCG) Using the Germicidal Spray Products as Disinfectants Test
MB-25-02 (21 pp, 293.92 Kb) OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces
MB-26-00 (11 pp, 250.50 Kb) Neutralization of Microbicidal Activity using the OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces
MB-27-01 (20 pp, 298 Kb) Germicidal and Detergent Sanitizing Action of Disinfectants Test
MB-28-02 (14 pp, 763 Kb) Production of Clostridium difficile Spores for Use in Efficacy Evaluation of Antimicrobial Agents
MB-30-00 (9 pp, 175 Kb) Preparation of hard water and other diluents for antimicrobial products
MB-31-01 (13 pp, 287 Kb) Quantitative Disk Carrier Test Method (QCT-2) Modified for Testing Antimicrobial Products Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard, Non-porous Surfaces
MB-32-00 (24 pp, 506 Kb) Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard Non-porous Surfaces
MB-33-00 (28 pp, 690 Kb) Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Vegetative Bacteria on Inanimate, Hard, Non-porous Surfaces.

Procedures

The following Standard Operating Procedures are pertinent to specific equipment and practices used in the OPP Microbiology Laboratory. The SOPs describe the laboratory's standard practices for calibrating equipment (EQ), performing quality control procedures (QC), preparing reports (ADM), maintaining chain of custody of samples (COC), and assuring quality management practices (QA and QMP).

To request copies of SOPs, send an e-mail to Luisa Samalot. Include the SOP ID number and title. Methods will be sent by e-mail or shipped via US Postal Service, regular mail.

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Procedure ID Number
Standard Operating Procedure
ADM-01-04 (9 pp, 100.6Kb) Preparation and Review of Performance Reports for Efficacy Testing
ADM-02-05 (10 pp, 97.25 Kb) Preparation and Review of Standard Operating Procedures (SOPs)
ADM-03-04 (14 pp, 99.56 Kb) Records and Archives
ADM-04-03 (14 pp, 113.37 Kb) OPP Microbiology Laboratory Training
ADM-05-02 (10 pp, 98.37 Kb) Guidelines for Use and Maintenance of Laboratory Notebooks and Project Binders
ADM-06-01 (9 pp, 39.46 Kb) Preparation and Review of Performance Reports for Efficacy Testing
COC-01-06 (18 pp, 190 Kb) Disinfectant Sample Login, Tracking, and Disposal
EQ-01-06 (14 pp, 109.42 Kb) Calibration and Maintenance of pH Meters
EQ-02-06 (11 pp, 107.77 Kb) Maintenance and Calibration of Thermometers and Thermometer/Hygrometers
EQ-03-07 (7pp, 214 Kb) Calibration and Maintenance of Weigh Balances
EQ-04-05 (10 pp, 178.69 Kb) Use and Maintenance of the Beckman DU 520 Spectrophotometer
EQ-05-06 (5 pp, 76 Kb) Calibration and Maintenance of Timers
EQ-06-05 (10 pp, 119.11 Kb) Calibration of Kimble Class A Burets
EQ-08-04 (10 pp, 129.8 Kb) Verification of Volume Dispensed and Maintenance of Oxford Automatic Dispensor and Hamilton Microlab 500
EQ-09-02 (12 pp, 199 Kb) Use of Petrifilm and PetriScan®
EQ-10-01 (9 pp, 85 Kb) Use and Maintenance of Orbital Shakers
QA-01-04 (11 pp, 77.87 Kb) Quality Assurance Unit and its Functions
QA-04-05 (8 pp, 133 Kb) Preparation and Maintenance of the Master Schedule
QC-01-05 (18 pp, 145.42 Kb) Quality Assurance of Purified Water
QC-02-04 (8 pp, 133.08 Kb) Monitoring of Laboratories for Airborne Contaminants
QC-03-05 (15 pp, 58.95 Kb) Glass Washing and Detergent Residues Test
QC-05-06 (17 pp, 175.46 Kb) Monitoring Environmental Parameters of Incubators, Refrigerators, Freezers, Laboratories and Sample Storage Rooms
QC-06-05 (11 pp, 103.98 Kb) Use and Maintenance of Biological Safety Cabinets
QC-09-06 (12 pp, 225.86 Kb) Establishment of Control Numbers and Tracking of Laboratory Supplies
QC-13-05 (13 pp, 147.34 Kb) Performance Verification of Autoclaves
QC-18-05 (12 pp, 98 Kb) Sterility Assessment of Disinfectant Product Samples
QC-19-06 (14 pp, 202 Kb) Verification of Digital Pipettes
QC-21-02 (20 pp, 179.95 Kb) Determining the Presence of Microbial Contamination in Disinfectant Products
QC-22-02 (17 pp, 126 Kb) VITEK 2 Compact: Use, Maintenance and Quality Control Procedures
QC-23-01 (11 pp, 190 Kb) Performance Verification of the Yamato Drying Oven (Mechanical Convection Oven)

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