![[logo] US EPA](http://www.epa.gov/epafiles/images/logo_epaseal.gif){kind=logo}
Microbiology Laboratory Antimicrobial Testing Methods & Procedures
You will need Adobe Reader to view some of the files on this page. See EPA's PDF page to learn more.
Methods
| Method ID Number |
Title |
|---|---|
| MB-01-05 (27 pp, 219 Kb) | Biosafety in the Laboratory |
| MB-02-03 (181 pp, 199 Kb) | Test Microbes for the AOAC Use-Dilution Method, AOAC Germicidal Spray Products Test, AOAC Confirmatory Tuberculocidal Test, AOAC Sporicidal Activity Test Method, and the Quantitative Suspension Test Method: Culture Initiation, Culture Maintenance and Quality Control |
| MB-02-04 (7 pp, 65.25 Kb) | Tracking of Test Microorganisms |
| MB-03-04 (14 pp, 203 Kb) | Screening Carriers Used in Disinfectant Efficacy Testing |
| MB-04-05 (14 pp, 166.15 Kb) | Enumeration of Bacterial Inocula on Carriers (Carrier Counts) for the Germicidal Spray Products as Disinfectants Test, Disinfectant Towelette Test, and the Tuberculocidal Activity of Disinfectants Test |
| MB-05-06 (34 pp, 298 Kb ) | AOAC Use Dilution Method for Testing Disinfectants |
| MB-06-03 (32 pp, 237 Kb) | Germicidal Spray Products as Disinfectants: Testing of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, and Mycobacterium bovis (BCG) |
| MB-07-04 (24 pp, 225 Kb) | Tuberculocidal Activity of Disinfectants: II. Confirmative in vitro Test for Determining Tuberculocidal Activityy |
| MB-09-02 (31 pp, 540Kb) | Disinfectant Towelette Test Against Staphylococcus aureus and Pseudomonas aeruginosa |
| MB-11-02 (25 pp, 206.8 Kb) | Neutralization Confirmation Assay For Disinfectant Products Tested Against Mycobacterium bovis (BCG) |
| MB-13-01 (17 pp, 101.3 Kb) | Handling Spills of Biohazardous Material |
| MB-15-01 (36 pp, 682 Kb) | AOAC Sporicidal Activity Test (Bacillus Species) |
| MB-16-01 (31 pp, 456.27 Kb) | Quantitative Suspension Test Method for Determining Tuberculocidal Efficacy of Disinfectants Against Mycobacterium bovis (BCG) AOAC Use Dilution Method and the AOAC Germicidal Spray Products as Disinfectants Test (Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica) |
| MB-17-01 (20 pp, 183.23 Kb) | Neutralization Confirmation Procedure for Products Evaluated with the AOAC Use Dilution Test and the AOAC Germicidal Spray Product Test (Staphylococcus aureus and Pseudomonas aeruginosa) |
| MB-18-00 (18 pp, 201 Kb) | Biosafety Practices for Handling Select Agent Samples in Room D122 |
| MB-21-00 (32 pp, 368.25 Kb) | Quantitative Three Step Method for Measuring the Efficacy of Liquid Sporicides against Spores of Bacillus subtilis on a Hard Nonporous Surface |
| MB-22-00 (12 pp, 162.75 Kb) | Disinfectant Sample Preparation |
Procedures
The following Standard Operating Procedures are pertinent to specific equipment and practices used in the OPP Microbiology Laboratory. The SOPs describe the laboratory's standard practices for calibrating equipment (EQ), performing quality control procedures (QC), preparing reports (ADM), maintaining chain of custody of samples (COC), and assuring quality management practices (QA and QMP).
To request copies of SOPs, send an e-mail to Luisa Samalot. Include the SOP ID number and title. Methods will be sent by e-mail or shipped via US Postal Service, regular mail.
| Procedure ID Number |
Standard Operating Procedure |
|---|---|
| ADM-01-03 (11 pp, 172.6Kb) | Preparation and Review of Disinfectant Performance Reports for Antimicrobial Testing Program |
| ADM-02-03 (16 pp, 95 Kb) | Preparation and Review of Standard Operating Procedures (SOPs) |
| ADM-03-02 (7 pp, 52 Kb) | Records and Archives |
| ADM-04-02 (14 pp, 116.90 Kb) | OPP Microbiology Laboratory Training Program |
| ADM-05-01 (11 pp, 52.5 Kb) | Guidelines for Use and Maintenance of Laboratory Notebooks and Project Binders |
| COC-01-04 (14 pp, 341.20 Kb) | Disinfectant Sample Login and Tracking |
| EQ-01-05 (14 pp, 156 Kb) | Calibration and Maintenance of pH Meters |
| EQ-03-04 (9 pp, 95 Kb) | Calibration and Maintenance of Weigh Balances |
| EQ-02-04 (8 pp, 55.3 Kb) | Calibration of Thermometers |
| EQ-04-03 (7 pp, 56 Kb) | Systems Check for the Beckman (DU Series 500) Spectrophotometer |
| EQ-05-04 (6 pp, 33.7 Kb) | Calibration and Maintenance of Timers |
| EQ-06-04 (10 pp, 167 Kb) | Calibration of Kimble Class A Burets |
| EQ-08-04 (10 pp, 129.8 Kb) | Verification of Volume Dispensed and Maintenance of Oxford Automatic Dispensor and Hamilton Microlab 500 |
| EQ-09-00 (14 pp, 344 Kb) | Use of Petrifilm and PetriScan® for Research Applications |
| EQ-10-00 (9 pp, 81.42 Kb) | Use and Maintenance of Orbital Shakers |
| QA-01-04 (11 pp, 77.87 Kb) | Quality Assurance Unit and its Functions |
| QA-04-03 (11 pp, 66.5 Kb) | Master Schedule Preparation |
| QC-01-04 (19 pp, 159 Kb) | Quality Assurance of Purified Water |
| QC-02-03 (17 pp, 431.6 Kb) | Air/Surface Monitoring of Microbiology Laboratories |
| QC-03-05 (15 pp, 75.9 Kb) | Glass Washing and Detergent Residues Test |
| QC-05-05 (17 pp, 309 Kb) | Monitoring Environmental Parameters of Incubators, Refrigerators, Freezers, Laboratories and Sample Storage Rooms |
| QC-06-04 (10 pp, 63 Kb) | Use and Maintenance of Biological Safety Cabinets |
| QC-08-04 (7 pp, 69 Kb) | Monitoring Temperature/Humidity of the Sample Storage Room |
| QC-09-04 (11 pp, 117.72 Kb) | Establishment of Control Numbers and Tracking Laboratory Supplies |
| QC-10-03 (9 pp, 103 Kb) | Media and Reagents: Examination and Expiration Time |
| QC-11-03 (16 pp, 229 Kb) | Performance Assessment and Sterility Verification of Prepared Media and Reagents |
| QC-13-04 (13 pp, 186 Kb) | Performance Verification of Autoclaves |
| QC-14-04 (6 pp, 47.6 Kb) | Monitoring Temperature of Water Baths for Holding (Tempering) Media |
| QC-15-03 (8 pp, 75 Kb) | Media and Reagent Preparation: Assigning Prep and Sterilization Run Numbers |
| QC-18-04 (12 pp, 98 Kb) | Sterility Assessment of Disinfectant Product Samples |
| QC-19-04 (14 pp, 145 Kb) | Verification of Digital Pipettes Using the PCS 2 Pipette Calibration System System |
| QC-21-00 (17 pp, 206 Kb) | Use of the AOAC Use Dilution Test and the Germicidal Spray Products Test without Test Microbes to Determine the Presence of Microbial Contamination in EPA-Registered Hospital Disinfectants |
| QC-22-00 (15 pp, 90 Kb) | VITEK 2 Compact: Use, Maintenance and Quality Control Procedures |
| QC-23-00 (11 pp, 166 Kb) | Performance Verification of the Yamato Drying Oven (Mechanical Convection Oven) |
| QMP-2007 (35 pp, 188.2 Kb) | Quality Assurance Management Plan for the Office of Pesticide Programs Microbiology Laboratory at the Environmental Science Center Fort Meade, Maryland |