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Draft OPPTS Test Guidelines
The DRAFT guidelines on this page are part of a series of test guidelines that have been developed by the Office of Prevention, Pesticides, and Toxic Substances (OPPTS) for use in the testing of pesticides and toxic substances, and the development of test data for submission to the Agency for review. This is not intended to be a comprehensive compilation of ALL draft guidelines, just those harmonized guidelines that OPPTS issued in draft for public comment. More information about OPPTS Harmonized Test Guidelines.
Although these draft guidelines are not yet available in final form, you may consult these guidelines if you are developing a proposed study protocol. However, before you use a draft guideline to generate data that will be submitted to EPA, please check with the appropriate EPA office.
A compilation of the harmonized guidelines that have been issued in draft, but have not yet issued in final form, is included at the end of the Master List (PDF) (29 pp, 70K, About PDF) of the OPPTS Harmonized Test Guidelines is available | Microsoft Excel Version (244K) (Excel viewer 
). The draft guidelines on this page are grouped based on whether the comment period on the draft is open.
You will need the free Adobe Reader to view some of the files on this page. See EPA's PDF page to learn more.
Draft Guidelines – Open for Public Review and Comment
Currently no draft harmonized test guidelines are open for public comment.
Draft Guidelines – Closed to Comment
810.3700 - Insect Repellents For Human Skin and Outdoor Premises (PDF) (13 pp, 38K)
850.1000 - Special Considerations for Conducting Aquatic Laboratory Studies (PDF) (13 pp, 45K)
850.1010 - Aquatic Invetebrate Acute Toxicity, Test, Freshwater Daphnids (PDF) (10 pp, 36K)
850.1020 - Gammarid Acute Toxicity Test (PDF) (11 pp, 36K)
850.1025 - Oyster Acute Toxicity Test (Shell Deposition) (PDF) (9 pp, 32K)
850.1035 - Mysid Acute Toxicity Test (PDF) (10 pp, 34K)
850.1045 - Penaeid Acute Toxicity Test (PDF) (9 pp, 32K)
850.1055 - Bivalve Acute Toxicity Test (Embryo Larval) (PDF) (7 pp, 27K)
850.1075 - Fish Acute Toxicity Test, Freshwater And Marine (PDF) (13 pp, 45K)
850.1085 - Fish Acute Toxicity Mitigated By Humic Acid (PDF) (10 pp, 35K)
850.1300 - Daphnid Chronic Toxicity Test (PDF) (12 pp, 42K)
850.1350 - Mysid Chronic Toxicity Test (PDF) (10 pp, 36K)
850.1400 - Fish Early-Life Stage Toxicity Test (PDF) (15 pp, 66K)
850.1500 - Fish Life Cycle Toxicity (PDF) (4 pp, 16K)
850.1710 - Oyster BCF (PDF) (14 pp, 50K)
850.1730 - Fish BCF (PDF) (25 pp, 74K)
850.1735 - Whole Sediment Acute Toxicity Invertebrates, Freshwater (PDF) (19 pp, 65K)
850.1740 - Whole Sediment Acute Toxicity Invertebrates, Marine (PDF) (14 pp, 50K)
850.1790 - Chironomid Sediment Toxicity Test (PDF) (16 pp, 57K)
850.1800 - Tadpole/Sediment Subchronic Toxicity Test (PDF) (15 pp, 49K)
850.1850 - Aquatic Food Chain Transfer (PDF) (4 pp, 16K)
850.1900 - Generic Freshwater Microcosm Test, Laboratory (PDF) (28 pp, 76K)
850.1925 - Site-Specific Aquatic Microcosm Test, Laboratory (PDF) (21 pp, 91K)
850.1950 - Field Testing For Aquatic Organisms (PDF) (7 pp, 21K)
850.2100 - Avian Acute Oral Toxicity Test (PDF) (11 pp, 38K)
850.2200 - Avian Dietary Toxicity Test (PDF) (12 pp, 42K)
850.2300 - Avian Reproduction Test (PDF) (16 pp, 53K)
850.2400 - Wild Mammal Acute Toxicity (PDF) (5 pp, 18K)
850.2450 - Terrestrial (Soil-Core) Microcosm Test (PDF) (19 pp, 123K)
850.2500 - Field Testing For Terrestrial Wildlife (PDF) (43 pp, 115K)
850.3020 - Honey Bee Acute Contact Toxicity (PDF) (8 pp, 27K)
850.3030 - Honey Bee Toxicity of Residues on Foliage (PDF) (6 pp, 23K)
850.3040 - Field Testing for Pollinators (PDF) (5 pp, 18K)
850.4000 - Background-Nontarget Plant Testing (PDF) (15 pp, 50K)
850.4025 - Target Area Phytotoxicity (PDF) (15 pp, 51K)
850.4100 - Terrestrial Plant Toxicity, Tier I (Seedling Emergence) (PDF) (8 pp, 29K)
850.4150 - Terrestrial Plant Toxicity, Tier I (Vegetative Vigor) (PDF) (8 pp, 28K)
850.4200 - Seed Germination/Root Elongation Toxicity Test (PDF) (8 pp, 29K)
850.4225 - Seedling Emergence, Tier II (PDF) (10 pp, 36K)
850.4230 - Early Seedling Growth Toxicity Test (PDF) (9 pp, 33K)
850.4250 - Vegetative Vigor, Tier II (PDF) (10 pp, 35K)
850.4300 - Terrestrial Plants Field Study, Tier III (PDF) (8 pp, 27K)
850.4400 - Aquatic Plant Toxicity Test Using Lemna Spp., Tiers I and II (PDF) (10 pp, 36K)
850.4450 - Aquatic Plants Field Study, Tier III (PDF) (9 pp, 30K)
850.4600 - Rhizobium-Legume Toxicity (PDF) (14 pp, 73K)
850.4800 - Plant Uptake and Translocation Test (PDF) (13 pp, 35K)
850.5100 - Soil Microbial Community Toxicity Test (PDF) (11 pp, 46K)
850.5400 - Algal Toxicity, Tiers I and II (PDF) (11 pp, 42K)
850.6200 - Earthworm Subchronic Toxicity Test (PDF) (13 pp, 43K)
850.6800 - Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals (PDF) (9 pp, 37K)
850.7100 - Data Reporting for Environmental Chemistry Methods (PDF) (9 pp, 30K)
