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OPPTS Harmonized Test Guidelines
Series 850 Ecological Effects Test Guidelines -- Public Drafts
The harmonized test guidelines have been developed for use in the testing of pesticides and toxic substances, and the development of test data that must be submitted to the Agency for review under Federal regulations.
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850.1000 Special considerations for conducting aquatic laboratory studies (PDF) (13pp, 45KB)
850.1010 Aquatic invetebrate acute toxicity, test, freshwater daphnids (PDF) (10pp, 36KB)
850.1020 Gammarid acute toxicity test (PDF) (11pp, 36KB)
850.1025 Oyster acute toxicity test (shell deposition) (PDF) (9pp, 32KB)
850.1035 Mysid acute toxicity test (PDF) (10pp, 34KB)
850.1045 Penaeid acute toxicity test (PDF) (9pp, 32KB)
850.1055 Bivalve acute toxicity test (embryo larval) (PDF) (7pp, 27KB)
850.1075 Fish acute toxicity test, freshwater and marine (PDF) (13pp, 45KB)
850.1085 Fish acute toxicity mitigated by humic acid (PDF) (10pp, 35KB)
850.1300 Daphnid chronic toxicity test (PDF) (12pp, 42KB)
850.1350 Mysid chronic toxicity test (PDF) (10pp, 36KB)
850.1400 Fish early-life stage toxicity test (PDF) (15pp, 66KB)
850.1500 Fish life cycle toxicity (PDF) (4pp, 16KB)
850.1710 Oyster BCF (PDF) (14pp, 50KB)
850.1730 Fish BCF (PDF) (25pp, 74KB)
850.1735 Whole sediment acute toxicity invertebrates, freshwater (PDF) (19pp, 65KB)
850.1740 Whole sediment acute toxicity invertebrates, marine (PDF) (14pp, 50KB)
850.1790 Chironomid sediment toxicity test (PDF) (16pp, 57KB)
850.1800 Tadpole/sediment subchronic toxicity test (PDF) (15pp, 49KB)
850.1850 Aquatic food chain transfer (PDF) (4pp, 16KB)
850.1900 Generic freshwater microcosm test, laboratory (PDF) (28pp, 76KB)
850.1925 Site-specific aquatic microcosm test, laboratory (PDF) (21pp, 91KB)
850.1950 Field testing for aquatic organisms (PDF) (7pp, 21KB)
850.2100 Avian acute oral toxicity test (PDF) (11pp, 38KB)
850.2200 Avian dietary toxicity test (PDF) (12pp, 42KB)
850.2300 Avian reproduction test (PDF) (16pp, 53KB)
850.2400 Wild mammal acute toxicity (PDF) (5pp, 18KB)
850.2450 Terrestrial (soil-core) microcosm test (PDF) (19pp, 123KB)
850.2500 Field testing for terrestrial wildlife (PDF) (43pp, 115KB)
850.3020 Honey bee acute contact toxicity (PDF) (8pp, 27KB)
850.3030 Honey bee toxicity of residues on foliage (PDF) (6pp, 23KB)
850.3040 Field testing for pollinators (PDF) (5pp, 18KB)
850.4000 Background-Nontarget plant testing (PDF) (15pp, 50KB)
850.4025 Target area phytotoxicity (PDF) (15pp, 51KB)
850.4100 Terrestrial plant toxicity, Tier I (seedling emergence) (PDF) (8pp, 29KB)
850.4150 Terrestrial plant toxicity, Tier I (vegetative vigor) (PDF) (8pp, 28KB)
850.4200 Seed germination/root elongation toxicity test (PDF) (8pp, 29KB)
850.4225 Seedling emergence, Tier II (PDF) (10pp, 36KB)
850.4230 Early seedling growth toxicity test (PDF) (9pp, 33KB)
850.4250 Vegetative vigor, Tier II (PDF) (10pp, 35KB)
850.4300 Terrestrial plants field study, Tier III (PDF) (8pp, 27KB)
850.4400 Aquatic plant toxicity test using Lemna spp. Tiers I and II (PDF) (10pp, 36KB)
850.4450 Aquatic plants field study, Tier III (PDF) (9pp, 30KB)
850.4600 Rhizobium-legume toxicity (PDF) (14pp, 73KB)
850.4800 Plant uptake and translocation test (PDF) (13pp, 35KB)
850.5100 Soil microbial community toxicity test (PDF) (11pp, 46KB)
850.5400 Algal toxicity, Tiers I and II (PDF) (11pp, 42KB)
850.6200 Earthworm subchronic toxicity test (PDF) (13pp, 43KB)
850.6800 Modified activated sludge, respiration inhibition test for sparingly soluble chemicals (PDF) (9pp, 37KB)
850.7100 Data Reporting for Environmental Chemistry Methods (PDF) (9pp, 30KB)
