![[logo] US EPA](http://www.epa.gov/epafiles/images/logo_epaseal.gif){kind=logo}
Laboratory Standard Operating Procedures
Standard Operating Procedures (SOPs) provide guidance on policies and procedures associated with individual analytical processes. The written SOPs are specific to the EPA Region 9 Laboratory and describe the procedures, actual instrumentation and equipment used. The following table lists the SOPs used by the EPA Region 9 Laboratory. The table also provides a summary of all SOPs in HTML Format.
DISCLAIMER: Mention of trade names or commercial products does not constitute endorsement or recommendation for use
| SOP# | SOP TITLE | Method Summary |
| SAMPLE CONTROL | ||
| 110 | Sample Receipt, Login, and Storage | 110 |
| 112 | Sample Receiving in the Absence of the Sample Custodian | 112 |
| 115 | Chemical Preservation upon Receipt at the Laboratory | 115 |
| 125 | Aqueous Sample Disposal | 125 |
| 130 | Glassware Cleaning Procedures | 130 |
| 140 | Trace Metal Labware Cleaning Procedure | 140 |
| 150 | Soil and Sediment Homoginization | 150 |
| CHEMISTRY: ORGANIC SAMPLE AND STANDARDS PREPARATION | ||
| 205 | Preparation of Organic-free Method Blank Water | 205 |
| 212 | Extraction of Water Samples by Continuous Liquid-Liquid Extraction of Water Samples | 212 |
| 239 | Recycling Used Methylene Chloride | 239 |
| 250 | TCLP Extraction | 250 |
| 255 | Standards Preparation Volatile Organic Analysis | 255 |
| 260 | Gel-Permeation Chromatography (GPC) Clean-up | 260 |
| 275 | TPH Extraction Using CLLE | 275 |
| 280 | TPH Extraction Using ASE | 280 |
| 290 | Extraction of Soil Samples Using PFE (Pressurized Fluid Extraction - ASE) | 290 |
| CHEMISTRY: ORGANIC SAMPLE ANALYSIS | ||
| 305 | Volatile Organics Analysis | 305 |
| 307 | 1,4-Dioxane Analysis | 307 |
| 310 | Analysis of VOCs in Air Collected in Canisters | 310 |
| 312 | SUMMA Canister Leak Check and Cleaning | 312 |
| 315 | Semivolatile Organics Analysis | 315 |
| 330 | Pesticides & PCB (Water, Sediment, Soil) by GC | 330 |
| 335 | Polychlorinated Biphenyls (PCBs) by GC | 335 |
| 350 | Analysis of EDB and DBCP in Water | 350 |
| 354 | Volatile Organic Analysis (Method 524.2) | 354 |
| 380 | TPH/BTEX Analysis | 380 |
| 385 | TPH/DRO Analysis by GC/FID | 385 |
| CHEMISTRY: INORGANIC SAMPLE AND STANDARDS PREPARATION | ||
| 401 | Nepholometric Turbidity Measurement | 401 |
| 403 | Sample Preparation for Trace Metals Analysis by GFAA, ICP-MS, ICP-AES | 403 |
| 405 | Preparation of Solid Samples for GFAA & ICP-AES Analysis | 405 |
| 415 | Preparation of Samples for Mercury Analysis | 415 |
| 420 | Microwave Digestion of Solid Samples | 420 |
| 425 | Reductive Precipitation Preconcentration | 425 |
| 430 | Tissue Preparation and Homogenization | 430 |
| CHEMISTRY: INORGANIC SAMPLE ANALYSIS | ||
| 460 | Percent Solids Determination | 460 |
| 461 | Total Dissolved Solids 160.1 | 461 |
| 462 | Total Suspended Solids 160.2 | 462 |
| 463 | Settable Solids | 463 |
| 490 | Asbestos | 490 |
| 503 | Analysis of Solids by ICP | 503 |
| 505 | Metals Analysis by ICP | 505 |
| 507 | Trace Metals by ICP-MS Method 200.8 | 507 |
| 511 | Trace Metals by GFAA | 511 |
| 513 | Analysis of Solids by GFAA | 513 |
| 515 | Mercury Analysis by Cold Vapor Atomic Absorption | 515 |
| 517 | Analysis of Solid Samples for Mercury | 517 |
| 530 | Ion Chromatography-Method 300 | 530 |
| 531 | Anions Analysis in Drinking Water by IC Method 300.0 | 531 |
| 532 | Cyanide Analysis by Method 335.4 | 532 |
| 545 | Specific Conductance | 545 |
| 550 | TOC Analysis of Water by EPA Method 415.1 | 550 |
| 560 | Total Alkalinity by Titrimetric Method | 560 |
| 565 | Sulfide Analysis by Titration | 565 |
| 570 | Hardness, Total (mg/l as CaCO3) | 570 |
| 580 | PH by EPA Method 150.1 | 580 |
| 590 | Ammonia Analysis | 590 |
| 592 | Total Kjeldahl Nitrogen in Water | 592 |
| 594 | Analysis of Nitrite and Nitrate in Water | 594 |
| 596 | Total Phosphorus in Water | 596 |
| QUALITY ASSURANCE | ||
| 805 | Refrigerator temperature monitoring | 805 |
| 815 | Balance Calibration and Maintenance | 815 |
| 820 | Lab Discrepancy/Corrective Action Report Procedure | 820 |
| 825 | Deionized water monitoring | 825 |
| 830 | Notification of exceeding safe drinking water mcl | 830 |
| 835 | Chromatographic integration procedures | 835 |
| 840 | Notebook documentation and control | 840 |
| 845 | Final Chemistry Data Review & Report Preparation | 845 |
| 846 | Internal Laboratory EPA Review of ESAT & EPA Generated Data | 846 |
| 850 | Preparation and usage of SOPs | 850 |
| 870 | Processing & reporting of Performance Evaluation samples | 870 |
| 880 | Initial & continuing demonstrations of proficiency (Chemistry) | 880 |
| 885 | Initial & continuing demonstrations of proficiency (Biology) | 885 |
| 890 | Calibration & maintenance of automatic pipets | 890 |
| BIOLOGY: MARINE TECHNICAL | ||
| 1000 | Suspended particulate phase-drilling mud toxicity using Mysidopsis bahia | 1000 |
| 1001 | Sea urchin & sand dollar fertilization toxicity test | 1001 |
| 1002 | Sea urchin & sand dollar larval development test | 1002 |
| 1003 | Marine sediment toxicity test using amphipods | 1003 |
| 1004 | Red abalone larval development toxicity test | 1004 |
| BIOLOGY: FRESHWATER TECHNICAL - CHRONIC | ||
| 1020 | Fathead minnow, larval survival & growth test | 1020 |
| 1021 | Daphnid, survival & reproduction test | 1021 |
| 1022 | Green alga, growth test | 1022 |
| 1023 | Daphnid, survival & growth test | 1023 |
| 1024 | Daphnid, survival & reproduction test | 1024 |
| BIOLOGY: FRESHWATER TECHNICAL - ACUTE | ||
| 1030 | Fathead minnow acute survival test | 1030 |
| 1031 | Ceriodaphnia dubia acute survival test | 1031 |
| 1032 | Daphnia magna acute survival test | 1032 |
| 1033 | Daphnia pulex acute survival test | 1033 |
| 1034 | Hyalella azteca, 7-d survival test for sediments | 1034 |
| BIOLOGY: GENERAL ACTIVITY | ||
| 1060 | Calibration and Operation of pH meter | 1060 |
| 1061 | Calibration and Operation of Dissolved Oxygen Meters | 1061 |
| 1063 | Calibration and Operation of Conductivity Meters | 1063 |
| 1067 | Calibration and Operation of Refractometers | 1067 |
| 1072 | Calibration & Operation of Cahn C-32 microbalance | 1072 |
| 1073 | Calibration & Operation of Oakton Portable pH/CON 10 Series Meter | 1073 |
| 1080 | Preparation of standard dilution water (freshwater) | 1080 |
| 1081 | Preparation of foods (freshwater) | 1081 |
| 1082 | Culturing of selenastrum capricornatum | 1082 |
| 1083 | Feeding of freshwater organisms | 1083 |
| 1084 | Freshwater amphipod culture maintenance | 1084 |
| 1085 | Glass & plasticware cleaning for marine tox. Testing | 1085 |
| 1086 | Kelp collection, preparation & feeding | 1086 |
| BIOLOGY: MICROBIOLOGY | ||
| 1101 | Membrane filtration coliform analysis | 1101 |
| 1102 | Multiple-tube fermentation analysis for total and fecal coliform | 1102 |
| 1103 | Colilert coliform and E. coli water analysis | 1103 |
| 1104 | Presence/absence coliform analysis | 1104 |
| 1105 | Routine sampling and analysis of water for bacteria, nitrates, lead and copper | 1105 |
| 1106 | Volunteer monitoring of surface waters for bacteria | 1106 |
| 1107 | Enterolert entercocci water analysis | 1107 |
| 1108 | Heterotrophic plate count for bacteria in water | 1108 |
| BIOLOGY: MISCELLANEOUS BIOLOGY | ||
| 1130 | Ash free dry mass-sediment | 1130 |
| 1131 | Ash free dry mass-periphyton | 1131 |
| 1133 | Biochemical oxygen demand-BOD | 1133 |
| 1134 | Chlorophyll-a (Method APHA 10200H) | 1134 |
| 1135 | Total & free residual chlorine - Hach DPD | 1135 |
| 1138 | Marine infaunal sample preparation and sorting | 1138 |
| CHEMISTRY: MISCELLANEOUS ANALYTICAL | ||
| 1304 | Immunoassay (ELISA) method for diazinon detection | 1304 |