Region 9 Laboratory Standard Operating Procedures

Standard Operating Procedures (SOPs) provide guidance on policies and procedures associated with individual analytical processes. The written SOPs are specific to the EPA Region 9 Laboratory and describe the procedures, actual instrumentation and equipment used. The following table lists the SOPs used by the EPA Region 9 Laboratory. The table also provides a summary of all SOPs in HTML Format.

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SOP#	SOP TITLE	Method Summary
SAMPLE CONTROL
110	Sample Receipt, Login, and Storage	110
112	Sample Receiving in the Absence of the Sample Custodian	112
115	Chemical Preservation upon Receipt at the Laboratory	115
125	Aqueous Sample Disposal	125
130	Glassware Cleaning Procedures	130
140	Trace Metal Labware Cleaning Procedure	140
150	Soil and Sediment Homoginization	150
CHEMISTRY: ORGANIC SAMPLE AND STANDARDS PREPARATION
205	Preparation of Organic-free Method Blank Water	205
212	Extraction of Water Samples by Continuous Liquid-Liquid Extraction of Water Samples	212
239	Recycling Used Methylene Chloride	239
250	TCLP Extraction	250
255	Standards Preparation Volatile Organic Analysis	255
260	Gel-Permeation Chromatography (GPC) Clean-up	260
275	TPH Extraction Using CLLE	275
280	TPH Extraction Using ASE	280
290	Extraction of Soil Samples Using PFE (Pressurized Fluid Extraction - ASE)	290
CHEMISTRY: ORGANIC SAMPLE ANALYSIS
305	Volatile Organics Analysis	305
307	1,4-Dioxane Analysis	307
310	Analysis of VOCs in Air Collected in Canisters	310
312	SUMMA Canister Leak Check and Cleaning	312
315	Semivolatile Organics Analysis	315
330	Pesticides & PCB (Water, Sediment, Soil) by GC	330
335	Polychlorinated Biphenyls (PCBs) by GC	335
350	Analysis of EDB and DBCP in Water	350
354	Volatile Organic Analysis (Method 524.2)	354
380	TPH/BTEX Analysis	380
385	TPH/DRO Analysis by GC/FID	385
CHEMISTRY: INORGANIC SAMPLE AND STANDARDS PREPARATION
401	Nepholometric Turbidity Measurement	401
403	Sample Preparation for Trace Metals Analysis by GFAA, ICP-MS, ICP-AES	403
405	Preparation of Solid Samples for GFAA & ICP-AES Analysis	405
415	Preparation of Samples for Mercury Analysis	415
420	Microwave Digestion of Solid Samples	420
425	Reductive Precipitation Preconcentration	425
430	Tissue Preparation and Homogenization	430
CHEMISTRY: INORGANIC SAMPLE ANALYSIS
460	Percent Solids Determination	460
461	Total Dissolved Solids 160.1	461
462	Total Suspended Solids 160.2	462
463	Settable Solids	463
490	Asbestos	490
503	Analysis of Solids by ICP	503
505	Metals Analysis by ICP	505
507	Trace Metals by ICP-MS Method 200.8	507
511	Trace Metals by GFAA	511
513	Analysis of Solids by GFAA	513
515	Mercury Analysis by Cold Vapor Atomic Absorption	515
517	Analysis of Solid Samples for Mercury	517
530	Ion Chromatography-Method 300	530
531	Anions Analysis in Drinking Water by IC Method 300.0	531
532	Cyanide Analysis by Method 335.4	532
545	Specific Conductance	545
550	TOC Analysis of Water by EPA Method 415.1	550
560	Total Alkalinity by Titrimetric Method	560
565	Sulfide Analysis by Titration	565
570	Hardness, Total (mg/l as CaCO3)	570
580	PH by EPA Method 150.1	580
590	Ammonia Analysis	590
592	Total Kjeldahl Nitrogen in Water	592
594	Analysis of Nitrite and Nitrate in Water	594
596	Total Phosphorus in Water	596
QUALITY ASSURANCE
805	Refrigerator temperature monitoring	805
815	Balance Calibration and Maintenance	815
820	Lab Discrepancy/Corrective Action Report Procedure	820
825	Deionized water monitoring	825
830	Notification of exceeding safe drinking water mcl	830
835	Chromatographic integration procedures	835
840	Notebook documentation and control	840
845	Final Chemistry Data Review & Report Preparation	845
846	Internal Laboratory EPA Review of ESAT & EPA Generated Data	846
850	Preparation and usage of SOPs	850
870	Processing & reporting of Performance Evaluation samples	870
880	Initial & continuing demonstrations of proficiency (Chemistry)	880
885	Initial & continuing demonstrations of proficiency (Biology)	885
890	Calibration & maintenance of automatic pipets	890
BIOLOGY: MARINE TECHNICAL
1000	Suspended particulate phase-drilling mud toxicity using Mysidopsis bahia	1000
1001	Sea urchin & sand dollar fertilization toxicity test	1001
1002	Sea urchin & sand dollar larval development test	1002
1003	Marine sediment toxicity test using amphipods	1003
1004	Red abalone larval development toxicity test	1004
BIOLOGY: FRESHWATER TECHNICAL - CHRONIC
1020	Fathead minnow, larval survival & growth test	1020
1021	Daphnid, survival & reproduction test	1021
1022	Green alga, growth test	1022
1023	Daphnid, survival & growth test	1023
1024	Daphnid, survival & reproduction test	1024
BIOLOGY: FRESHWATER TECHNICAL - ACUTE
1030	Fathead minnow acute survival test	1030
1031	Ceriodaphnia dubia acute survival test	1031
1032	Daphnia magna acute survival test	1032
1033	Daphnia pulex acute survival test	1033
1034	Hyalella azteca, 7-d survival test for sediments	1034
BIOLOGY: GENERAL ACTIVITY
1060	Calibration and Operation of pH meter	1060
1061	Calibration and Operation of Dissolved Oxygen Meters	1061
1063	Calibration and Operation of Conductivity Meters	1063
1067	Calibration and Operation of Refractometers	1067
1072	Calibration & Operation of Cahn C-32 microbalance	1072
1073	Calibration & Operation of Oakton Portable pH/CON 10 Series Meter	1073
1080	Preparation of standard dilution water (freshwater)	1080
1081	Preparation of foods (freshwater)	1081
1082	Culturing of selenastrum capricornatum	1082
1083	Feeding of freshwater organisms	1083
1084	Freshwater amphipod culture maintenance	1084
1085	Glass & plasticware cleaning for marine tox. Testing	1085
1086	Kelp collection, preparation & feeding	1086
BIOLOGY: MICROBIOLOGY
1101	Membrane filtration coliform analysis	1101
1102	Multiple-tube fermentation analysis for total and fecal coliform	1102
1103	Colilert coliform and E. coli water analysis	1103
1104	Presence/absence coliform analysis	1104
1105	Routine sampling and analysis of water for bacteria, nitrates, lead and copper	1105
1106	Volunteer monitoring of surface waters for bacteria	1106
1107	Enterolert entercocci water analysis	1107
1108	Heterotrophic plate count for bacteria in water	1108
BIOLOGY: MISCELLANEOUS BIOLOGY
1130	Ash free dry mass-sediment	1130
1131	Ash free dry mass-periphyton	1131
1133	Biochemical oxygen demand-BOD	1133
1134	Chlorophyll-a (Method APHA 10200H)	1134
1135	Total & free residual chlorine - Hach DPD	1135
1138	Marine infaunal sample preparation and sorting	1138
CHEMISTRY: MISCELLANEOUS ANALYTICAL
1304	Immunoassay (ELISA) method for diazinon detection	1304

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