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R9 Laboratory SOP 1023
DAPHNID, DAPHNIA MAGNA, SURVIVAL AND GROWTH TEST
Summary
This SOP describes methods for measuring the chronic, short-term adverse effects of effluents or receiving waters on cladoceran, }{\i\f3\fs24 Daphnia magna }{\fs24 during 2-day to 4-day static-renewal exposures. This method has been written following the draft method outlined in the summary status report entitled "Status Report on 4-Day Daphnia magna Growth Method Test Results". These procedures are also suitable to freshwater containing chemicals of known or unknown dilutions.
Daily observations on mortality permit the calculation of acute toxicity at other exposure periods(i.e. 24-h, 48 h)
Daphnia magna neonates are exposed in a static renewal system to different concentrations of effluents, or receiving waters for 48 or 96 hour periods. Test results are based on survival and growth, as measured by weight of the surviving organisms.