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R9 Laboratory SOP 1024
DAPHNID, DAPHNIA MAGNA, SURVIVAL AND REPRODUCTION TEST
Summary
This SOP describes methods for measuring the chronic, short-term adverse effects of effluents or receiving waters on cladoceran, Daphnia magna during a 7-day static-renewal exposure. This method has been written following the EPA method entitled "A Short-Term Chronic Toxicity Test Using Daphnia Magna", EPA/600/D-87/080, March 1987.
These procedures are also suitable to freshwater containing chemicals of known or unknown dilutions. Daily observations on mortality permit the calculation of acute toxicity at other exposure periods(i.e. 24-h, 48 h)
Ten-day old Daphnia magna are exposed in a static renewal system to different concentrations of effluents, or receiving waters for a seven day period. Test results are based on survival and reproduction, as measured by the number of young produced per surviving adult.