![[logo] US EPA](http://www.epa.gov/epafiles/images/logo_epaseal.gif){kind=logo}
R9 Laboratory SOP 1032
DAPHNID, DAPHNIA MAGNA, ACUTE SURVIVAL TEST
Summary
This SOP describes methods for measuring the acute, short-term adverse effects of effluents or receiving waters on cladoceran, Daphnia magna during 2-day or 4-day static or static-renewal exposures. This method has been written following the EPA method outlined in the manual, EPA/600/4-90/027F, Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms (Fourth Edition).
These procedures are also suitable to freshwater containing chemicals of known or unknown dilutions. Daily observations on mortality permit the calculation of acute toxicity at other exposure periods(i.e. 24-h, 48-h)
Daphnia magna neonates are exposed in a static or static renewal system to different concentrations of effluents, or receiving waters for 48 or 96 hour periods. Test results are based on survival.