Region 8
Whole Effluent Toxicity (WET) Useful Documents
National Information
Regional Information
EPA Regions 8, 9 and 10 toxicity training tool (TTT). January 2010.
"Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms" Fifth Edition. EPA 821/R-02/012, U.S. EPA, October 2002
"Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms" Fourth Edition. EPA 821/R-02/013 U.S. EPA, October 2002
Whole Effluent Toxicity Reporting Forms
These report forms are provided as the suggested format for State Whole Effluent Toxicity reporting. They can be modified and updated to reflect individual laboratory practices, but at a minimum should maintain the contents specified within. For electronic copies, please contact Donna Roberts at 303-312-6371.
Ceriodaphnia dubia ACUTE TOXICITY TEST REPORT FORMAT
Ceriodaphnia dubia CHRONIC TOXICITY TEST REPORT FORMAT
"Technical Support Document for Water Quality Based Toxics Control" Second Printing, EPA 505/2-90-001, U.S. EPA, March 1991.
"Methods for Aquatic Toxicity Identification Evaluations, Phase I Toxicity Characterization Procedures", Second Edition, EPA/600/6-91/003, February 1991.
"Methods for Aquatic Toxicity Identification Evaluations, Phase II Toxicity Identification Procedures for Samples Exhibiting Acute and Chronic Toxicity", EPA/600/R-92/080, September 1993
"Methods for Aquatic Toxicity Identification Evaluations, Phase III Toxicity Confirmation Procedures for Samples Exhibiting Acute and Chronic Toxicity",EPA/600/R-92 /081, September 1993.
"Toxicity Identification Evaluation: Characterization of Chronically Toxic Effluents, Phase I", EPA 600/6-91/005F, May 1992.
"Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination System" EPA 833-R-00-003, June 2000
"Toxicity Reduction Evaluation Guidance for Municipal Wastewater Treatment Plants", EPA/833B-99/002, August 1999
"Generalized Methodologv for Conducting Industrial Toxicity Reduction Evaluations (TREs)", EPA/600/2-88/070, April 1989