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Fish Short-Term Reproduction (Fish Screen) Assay Peer Review Materials
EPA announced in a July 2007 Federal Register Notice (PDF) (4 pp, 67K, About PDF) the approach it intends to take for conducting peer reviews of the Tier 1 screening assays and Tier 2 testing assays that are being validated. As described in the Federal Register Notice, peer review materials will be posted as they become available. Materials may include the documents to be peer reviewed, background documents, the charge to the peer reviewers, and reports that summarize the results of peer reviews. Peer review materials for the Fish Screen Assay can be accessed below:
You will need the free Adobe Reader to view some of the files on this page. See EPA's PDF page to learn more. If you need help accessing these PDF documents, please contact William Wooge at 202-564-8476 or wooge.william@epa.gov for assistance.
Charge to Peer Reviewers
Charge to Peer Reviewers (PDF) (2 pp, 14K)
Integrated Summary Report
Validation of the Fish Short-Term Reproduction Assay: Integrated Summary Report (PDF) (104 pp, 461K)
Attachment A: Endocrine Disruptor Screening Program Validation Paper
Validation of Screening and Testing Assays Proposed for the EDSP (PDF) (21pp, 131K)
Attachment B: OECD Fish Screening Assay Detailed Review Paper
Attachment C: OECD Fish Short-term Reproduction Assay Phase 1B Report
Attachment D: Revised Draft Final Report on Fathead Minnow Fish Screening Assay OECD Phase 1 B Follow-Up
Revised Draft Final Report on Fathead Minnow (Pimephales promelas) Fish Screening Assay OECD Phase 1 B Follow-Up (PDF) (656 pp, 51.2M)
Attachment E: US EPA Fish Short-term Reproduction Assay Inter-laboratory Study Report
DRAFT FINAL REPORT and 12 Corresponding Individual Laboratory Reports (ZIP) (13 PDF files, 113M)
Attachment F: Fish Short-term Reproduction Assay Protocol
Fish Short-Term Reproduction Assay Test Protocol – Fathead Minnow (PDF) (29 pp, 415K)
Attachment G: Short-term Test Method for Assessing the Reproductive Toxicity of Endocrine Disrupting Chemicals Using the Fathead Minnow
Attachment H: Histopathology Guidelines for the Fathead Minnow 21-day Reproduction Assay
Histopathology guidelines for the Fathead Minnow (Pimephales promelas) 21-day Reproduction Assay July 6, 2006 (PDF) (59 pp, 5.1M)
Background Documents
Reference 1: Reference 1 OECD Phase 2 Report: Draft Report of the Validation of the 21-Day Fish Endocrine Screening Assay for the Detection of Endocrine Active Substances (Phase 2-Testing Negative Substances)
Reference 2: Reference 2 OECD Guidance Document 23: Testing and Assessment: Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures
Reference 3: Fish Short-term Reproduction Assay Discussion Paper
Fish Screening Assay Discussion Paper 9/18/2006 U.S. EPA (PDF) (87 pp, 1.6M)
Peer Review Results
Peer Review Results for the Fish Short-Term Reproduction (Fish Screen) Assay (PDF) (110 pp, 491K)
EPA Response to Peer Review Comments (PDF) (26 pp, 249K)