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Biological Pollutants in Ambient Water

Guidelines Establishing Test Procedures for the Analysis of Pollutants; Analytical Methods for Biological Pollutants in Wastewater and Sewage Sludge (September 29, 2006)

We issued new methods that use culture-based approaches to detect enterococci and Escherichia coli (E. coli) in wastewater. Additional tests will also identify Salmonella and fecal coliform bacteria in sewage sludge.

You will need Adobe Acrobat Reader to view the Adobe PDF files on this page. See EPA's PDF page for more information about getting and using the free Acrobat Reader.

Number Method Title
1103.1 Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC) (PDF) (45 pages, 891 K)
1106.1 Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA) (PDF) (42 pages, 789 K)
1600 Membrane Filter Test Method for Enterococci in Water (PDF) (42 pages, 599 K)
1603 Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC) (PDF) (42 pages, 535 K)
1680 Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC Medium (PDF) (49 pp, 296 K)
1681 Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using A-1 medium (PDF) (51 pp, 341 K)
1682 Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium (PDF) (48 pp, 403 K)


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