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Cole, F.A., B.L. Boese, R.C. Swartz, J.O. Lamberson and T.H. DeWitt. 1999. Effect of sediment storage on the toxicity of sediments spiked with fluorathene to the amphipod Rhepoxynius abronius. Environmental Toxicology and Chemistry 19(3)744-748. NHEERL - COR-2304J

To determine the effect of storage on contaminant bioavailability and toxicity, two sediments types, a fine sand and a silty sand, were spiked with nine concentrations of fluoranthene (0.0-50 gm/dry kg) then stored at 4EC for up to 170d. Toxicity of the stored sediment was determined eight times during this storage interval using standard 10-d toxicity tests with the marine infaunal amphipod Rhepoxynius abronius. The concentrations of fluoranthene in the sediment and interstitial water were determined on samples taken on each test date. The toxicity of fluoranthene in the silty sand was similar for all storage times with LC50s ranging from 5.3 to 6.6 gm/g organic carbon (OC). The LC50 in the fine sand was 7.4 mg/g OC after 13 d of storage, ranged from 10.2 to 11.8 mg/g OC during 27 to 83 d of storage, and increased to 24.2 and 27.6 mg/g OC after 121 and 170 d of storage, respectively. These data indicate that the toxicity of both the fine and the silty sand remained essentially constant during storage from days 27 to 83. Toxicity tests conducted before or after that period may give misleading results because of disequilibrium or unknown storage effects.

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