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Schuytema, Gerald S., and A. V. Nebeker. 1998. Comparative toxicity of diuron on survival and growth of Pacific tree frog, bullfrog, red-legged frog, and African clawed frog embryos and tadpoles. Archives of Environmental Contamination and Toxicology 34:370-376.

The effects of the herbicide diuron on survival and growth of Pacific tree frog (Pseudacris regilla), bullfrog (Rana catesbeiana), red-legged frog (Rana aurora), and African clawed frog (Xenopus laevis) embryos and tadpoles were determined in static-renewal tests. P. regilla and X. laevis embryos had reduced growth and developed increased deformities in diuron concentrations over 20 mg/L. Hind limb bud and forelimb development were retarded in R. aurora following 14 days exposure to diuron concentration of >7.6 mg/L. Mean 14-day LC50s for P. regilla and X.laevis tadpoles were 15.2 and 11.3 mg/L diuron, respectively. The 21-day LC50 for R. catesbeiana tadpoles was 12.7 mg/L diuron. The 14-day LC50) for R. aurora tadpoles was 22.2 mg/L . The lowest NOAELs calculated in embryo tests were 14.5 mg/L for P. regilla (10 days) and 7.6 mg/L diuron for X. laevis (4 days). The lowest NOAELs calculated in tadpole tests were: P. regilla 14.5 mg/L (14 days); R. catesbeiana, 7.6 mg/L (21 days); R. aurora, 7.6 Mg/L (14 days) and X. Laevis, >29.1 mg/L (14 days). Diuron concentrations having an effect on survival, growth, and malformation in the laboratory were much higher than those found in normal field spray situations; field studies would be needed to determine the hazard to amphibians in areas of localized pooling of recently applied herbicide in the environment.

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