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EPA Region 9 Laboratory Standard Operating Procedures

Note: To obtain a copy of a specific standard operating procedure (SOP), please contact Barbara Bates (Bates.Barbara@epa.gov) at (510) 412 - 2325.

Category SOP# SOP TITLE
Administrative/Miscellaneous 010 Guidance for use of EPA Purchase Cards
Administrative/Miscellaneous 020 Shipping and Receiving of Coolers, Supplies and Equipment
Administrative/Miscellaneous 030 Sample Coordination by the RSCC
Administrative/Miscellaneous 050 State Drinking Water Laboratory Certification Program Oversight
Sample Control 110 Sample Receiving Procedure and Login
Sample Control 112 Sample Receiving in the Absence of the Sample Custodian
Sample Control 115 Chemical Preservation of Environmental Samples upon Receipt at the Laboratory
Sample Control 125 Sample Disposal
Sample Control 130 Glassware Cleaning Procedures
Sample Control 150 Soil and Sediment Homoginization
Organic Sample Preparation 205 Preparation of Organic-free Blank Water
Organic Sample Preparation 239 Procedure for Recycling Used Methylene Chloride
Organic Sample Preparation 260 Gel-Permeation Chromatography (GPC) Clean-up
Organic Sample Preparation 275 Extraction of Water Samples by Continuous Liquid-Liquid Extraction (CLLE)
Organic Sample Preparation 285 Soxhlet Extraction of Solid Samples
Organic Sample Preparation 290 Extraction of Soil Samples Using Pressurized Fluid 
Chemistry: Organic Sample Analysis 305 Volatile Organics Compundd Analysis in Solids
Organic Sample Analysis 307 VOCs by GCMS following desorption from Radiellp 130 passive diffusive samples
Organic Sample Analysis 311 Analysis of Volatile Organic Compounds in Air and Soil Vapor
Organic Sample Analysis 312 Cleaning and Certification of Specially Prepared Cannisters for Air Sampling
Organic Sample Analysis 314 TO15 SIM-Analysis of Volatile Organic Compounds in Air
Organic Sample Analysis 315 Semivolatile Organic Compound Analysis
Organic Sample Analysis 325 Determination of Dissolved Gases in Water
Organic Sample Analysis 330 Organicchlorine Pesticide Analysis by Gas Chromatography (GC)
Organic Sample Analysis 335 Polychlorinated Biphenyls (PCBs) by GC
Organic Sample Analysis 340 GC/MS-TOF Analysis of UDA of Ultra-Dilute Chemical Warfare Agents (UDA) Standards and Samples
Organic Sample Analysis 353 Low-level Volatiles
Organic Sample Analysis 354 Volatile Organic Compound Analysis in Water
Organic Sample Analysis 375 Low-level Semi-Volatile Organics Analysis
Organic Sample Analysis 380 Purgeable Hydrocarbons by GC PID/FID
Organic Sample Analysis 385 Extractable Petrleum Hydrocarbons by GC/FID
Organic Sample Preparation 250 TCLP Extraction
Organic Sample Preparation 252 California Waste Extraction Test (WET)
Organic Sample Preparation 254 SPLP Extraction
Organic Sample Preparation 256 Bioaccessibility Extraction
Inorganic Sample Preparation and Solids Analyses 401 Nepholometric Turbidity Measurement
Inorganic Sample  Preparation and Solids Analyses 403 Aqueous Sample Preparation for ICP-AES and ICP-MS
Inorganic Sample  Preparation and Solids Analyses 405 Preparation of Solid Samples for Metals Analysis
Inorganic Sample  Preparation and Solids Analyses 407 Preparation of Leachate Procedure Extracts for Metals Analysis
Inorganic Sample  Preparation and Solids Analyses 409 Digestion for Lead on Filters
Inorganic Sample  Preparation and Solids Analyses 430 Tissue Preparation and Homogenization
Inorganic Sample  Preparation and Solids Analyses 460 Percent Solids Determination
Inorganic Sample  Preparation and Solids Analyses 461 Analysis of Total Dissolved Solids
Inorganic Sample  Preparation and Solids Analyses 462 Analysis of Total Suspended Solids
Inorganic Sample  Preparation and Solids Analyses 463 Settleable Solids
Inorganic Sample  Preparation and Solids Analysis 464 Suspended Sediment Concentration
Inorganic Sample  Analysis 503 Determination of Trace Elements in Solids by ICP-AES
Inorganic Sample  Analysis 505 Determination of Trace Elements in Water by ICP-AES
Inorganic Sample  Analysis 507 Determination of Trace Elements in Water by ICP-MS
Inorganic Sample  Analysis 509 Determination of Lead on Filters by ICP/MS
Inorganic Sample  Analysis 515 Determination of Mercury in Water by CVAA Spectrometry
Inorganic Sample  Analysis 517 Analysis of Solid Samples for Mercury
Inorganic Sample  Analysis 525 Analysis of Hexavalent Chromium in Water by Ion Chromatography
Inorganic Sample  Analysis 530 Analysis of Anions by Ion Chromatography
Inorganic Sample  Analysis 531 Analysis of Perchlorate by Ion Chromatography
Inorganic Sample  Analysis 532 Cyanide Analysis
Inorganic Sample  Analysis 535 Analysis of Mercury in Solids by Thermal Decomposition and AAS
Inorganic Sample  Analysis 537 Analysis of Perchlorate by LC-MS-MS
Inorganic Sample  Analysis 545 Determination of Specific Conductance
Inorganic Sample  Analysis 553 Determination of TOC in Water by EPA Method 415.3
Inorganic Sample  Analysis 560 Total Alkalinity by Titrimetric Method
Inorganic Sample  Analysis 565 Sulfide by Titration
Inorganic Sample  Analysis 570 Total Hardness, Titrimetric Method
Inorganic Sample  Analysis 575 Determination of Ferrous Iron by Titration with Dichromate
Inorganic Sample  Analysis 580 pH Determination in Aqueous Samples
Inorganic Sample  Analysis 582 pH in Solid Samples
Inorganic Sample  Analysis 590 Ammonia Analysis
Inorganic Sample  Analysis 592 Analysis of Total Kjeldahl Nitrogen in Water
Inorganic Sample  Analysis 594 Analysis of Nitrite and Nitrate in Water
Inorganic Sample  Analysis 596 Analysis of Total Phosphorus in Water
Health, Safety & Environmental Compliance 704 Inspection Procedures for Emergency Eyewash and Shower
Health, Safety & Environmental Compliance 706 Laboratory Waste Management
Health, Safety & Environmental Compliance 712 Inspection Procedures for the Satellite and 90-Day Hazardous Waste
Health, Safety & Environmental Compliance 716 Cleaning and Recycling of Empty Glass Containers
Health, Safety & Environmental Compliance 730 Neutralization of Aqueous Wastes
Health, Safety & Environmental Compliance 745 Maintenance and Inspection of Spill Cleanup Equipment
Health, Safety & Environmental Compliance 747 Laboratory Chemical Inventory Maintenance
Health, Safety & Environmental Compliance 748 Chemical Adoption Program
Health, Safety & Environmental Compliance 750 Inspection Procedures for Fire Extinguishers
Health, Safety & Environmental Compliance 760 Maintenance and Upkeep of Radioactive Sources
Health, Safety & Environmental Compliance 765 Procedure for Spill Response and Exposure Control for Dichloromethane
Health, Safety & Environmental Compliance 770 Handling Cryogenic Materials
Health, Safety & Environmental Compliance 775 Handling and Disposal of Broken Glassware
Health, Safety & Environmental Compliance 780 Procedure for Chemical Spill Response and Cleanup
Health, Safety & Environmental Compliance 785 Procedure for Storage, Monitoring, and Handling of Peroxide Forming Chemicals
Quality Assurance 805 Refrigerator and Freezer temperature monitoring
Quality Assurance 810 Internal Audits of Laboratories
Quality Assurance 817 Accuracy Check of Laboratory Thermometers
Quality Assurance 820 Lab Discrepancy and Corrective Action Report Procedure
Quality Assurance 825 Deionized water monitoring
Quality Assurance 830 Notification Procedure for Results Exceeding the Safe Drinking Water Act Limits
Quality Assurance 835 Chromatographic Integration Procedure
Quality Assurance 840 Notebook Documentation and Control
Quality Assurance 845 Internal Data Review & Report Preparation
Quality Assurance 850 Preparation and Usage of Standard Operating Procedures
Quality Assurance 880 Demonstration of Capability
Quality Assurance 885 Initial & Continuing Demonstrations of Biological Laboratory Capability and Analysis Proficiency
Quality Assurance 890 Calibration & Maintenance of Automatic Pipettes
Quality Assurance 892 Initial Instrument Calibration Guidelines
Biological Testing 1001 Sea Urchin & Sand Dollar Fertilization Toxicity Test
Biological Testing 1004 Red Abalone, Larval Development Toxicity Test
Biological Testing 1060 Calibration and Operation of pH Meters
Biological Testing 1061 Calibration and Operation of Dissolved Oxygen Meters
Biological Testing 1063 Calibration and Operation of Conductivity Meters
Biological Testing 1067 Calibration and Operation of Refractometers
Biological Testing 1073 Calibration & Operation of Oakton Portable pH/CON 10 Series Meter
Biological Testing 1085 Glassware and Plasticware Cleaning for Toxicity Tests
Biological Testing 1086 Collection, Preparation,, and Feeding of Kelp for Sea Urchin and Abalone and Tank Cleaning
Microbiology and Miscellaneous Biology 1103 Colilert coliform and E. Coli Water Analysis
Microbiology and Miscellaneous Biology 1106 Volunteer Monitoring of Surface Waters for Bacteria
Microbiology and Miscellaneous Biology 1107 Enterolert Entercocci Water Analysis
Microbiology and Miscellaneous Biology 1108 Heterotrophic Plate Count for Bacteria in Water
Microbiology and Miscellaneous Biology 1109 Microbiology Autoclaves
Microbiology and Miscellaneous Biology 1131 Analysis of Ash Free Dry Mass
Microbiology and Miscellaneous Biology 1133 Biochemical Oxygen Demand
Microbiology and Miscellaneous Biology 1134 Chlorophyll-a (Method APHA  10200H)
Microbiology and Miscellaneous Biology 1135 Total & Fee Rsidual Clorine - Hach Dpd
Field Sampling and Analysis 1201 General Field Equipment Inventory, Documentation Maintenance, Calibration and Operation
Field Sampling and Analysis 1202 Operational Guidelines for Field Activities
Field Sampling and Analysis 1210 Calibration and Operation of  YS1 6600V2 Sonde
Field Sampling and Analysis 1211 Calibration and Operation of  YS1 556 Multi-Probes
Field Sampling and Analysis 1212 Calibration and Operation of the Manta 2 Sonde
Field Sampling and Analysis 1213 Calibration and Operation of YS1 EXO Sonde
Field Sampling and Analysis 1220 Groundwater Monitoring Well Sampling
Field Sampling and Analysis 1221 Soil Sampling
Field Sampling and Analysis 1222 Routine Sampling and Analysis of Drinking water
Field Sampling and Analysis 1223 Sediment Sampling
Field Sampling and Analysis 1224 Surface Water Sampling
Field Sampling and Analysis 1225 Soil Sampling for Volatile Organic Compounds
Field Sampling and Analysis 1227 Air Sampling for Volatile Organic Compounds
Microbiology and Miscellaneous Biology 1305 Microcystin by ELISA Analysis
Administrave/Miscellaneous 051 State Drinking Water Laboratory Certification
Organic Sample Preparation 230 Extraction of Ultra-Dilute Chemical Warfare Agents (UDA) from Aqueous Samples
Organic Sample Preparation 240 Exctraction of Ultra-Dilute Chemical Warfare Agents (UDA) from Wipe Samles
Organic Sample Preparation 242 Extraction of Ultra-Dilute Chemical Warfare Agents (UDA) from Soil Samples
Organic Sample Preparation 245 Accountability of Ultra-Dilute Chemical Warfare Agent Reference Solutions
Organic Sample Preparation 265 Extraction of Low-Level Phenols and Polycyclic Aromatic Hydrocarbons from Water
Organic Sample Analysis 342 GC/MS Single Qualdruple Analysis of UDA of Ultra-Dilute Chemical Warfare Agents (UDA) Standards and Samples
Inorganic Sample nalysis 534 Determination of Low Level Mercury in Water
Inorganic Sample  Analysis 536 Determination of Methyl Mercury in Water
Inorganic Sample  Analysis 561 Acidity
Quality Assurance 818 Accuracy Verification of Weights and Balances
Quality Assurance 893 Recertification of Standards and Reagents
Field Analytical Services (FASP) 903 Field Analytical Support Project (FASP) Mobilization of Field Lab
Field Analytical Services (FASP) 910 Analysis of Volatile Organic Compounds (VOCs) in Air by Field Portable GC/MS
Field Analytical Services (FASP) 940 Volatile Organic Compond Monitoring by ppb RAE Plus Portable Photoionization Detector
Field Sampling and Analysis 1230 Sampling Equipment Documentation
Field Sampling and Analysis 1250 Field Elemental Mercury in Air by Tekran 2537A, Reactive & Particulate by Tekran 1130/1135
Field Sampling and Analysis 1251 Precious Metals Analysis of Catalyst Washcoat using a Niton XL3t FPXRF Analyzer
Field Sampling and Analysis 1255 Temperature Calibration Verification for a FLIR GF320 Infrared Camera
Field Sampling and Analysis 1320 Field-Portable X-Ray Florescenc (FPXRF) Analyzer