ESAM Collaborative Analytical Methods and Protocols for Pathogens
EPA's Homeland Security Research Program (HSRP) has worked with partnering laboratories and agencies to develop several of the analytical methods and protocols addressing target chemical, radiochemical, pathogen and biotoxin contaminants of concern in environmental samples, as well as radiochemical contamination of outdoor building and infrastructure materials.
The methods developed by HSRP are a subset of the methods included in EPA's SAM 2022 and can also be accessed here.
Pathogen Methods
- "Protocol for Detection of Bacillus anthracis in Environmental Samples During the Remediation Phase of an Anthrax Event" (EPA BA Protocol). Cincinnati, OH: U.S. EPA. EPA/600/R-12/577.
- U.S. EPA. 2016. "Protocol for Detection of Yersinia pestis in Environmental Samples During the Remediation Phase of a Plague Incident" (EPA YP Protocol). Cincinnati, OH: U.S. EPA. EPA/600/R-16/109.
- U.S. EPA. September 2010. "Standard Analytical Protocol for Escherichia coli O157:H7 in Water" (EPA EC Protocol). Cincinnati, OH: U.S. EPA. EPA/600/R-10/056.
- U.S. EPA and CDC. 2011. "Comparison of Ultrafiltration Techniques for Recovering Biothreat Agents in Water" (EPA and CDC UF Report). Cincinnati, OH: U.S. EPA. EPA 600/R-11/103.
- U.S. EPA., Cincinnati OH, February 2017, Rev. 0, "Processing Protocol for Soil Samples Potentially Contaminated with Bacillus anthracis Spores," EPA/600/R-17/028.
- U.S. EPA. 2010. "Standard Analytical Protocol for Vibrio cholerae O1 and O139 in Drinking Water and Surface Water" (EPA VC Protocol). Washington, DC: U.S. EPA. EPA 600/R-10/139 (PDF). (56 pp, 1,000 K, October 2010)
- U.S. EPA and CDC 2022. "Protocol for Collection of Water Samples for Detection of Pathogens and Biothreat Agents," Washington, DC: U.S. EPA. EPA/600/R-21/280.