Skip to main content
U.S. flag

An official website of the United States government

Here’s how you know

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

HTTPS

Secure .gov websites use HTTPS
A lock (LockA locked padlock) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

    • Environmental Topics
    • Air
    • Bed Bugs
    • Cancer
    • Chemicals, Toxics, and Pesticide
    • Emergency Response
    • Environmental Information by Location
    • Health
    • Land, Waste, and Cleanup
    • Lead
    • Mold
    • Radon
    • Research
    • Science Topics
    • Water Topics
    • A-Z Topic Index
    • Laws & Regulations
    • By Business Sector
    • By Topic
    • Compliance
    • Enforcement
    • Laws and Executive Orders
    • Regulations
    • Report a Violation
    • Environmental Violations
    • Fraud, Waste or Abuse
    • About EPA
    • Our Mission and What We Do
    • Headquarters Offices
    • Regional Offices
    • Labs and Research Centers
    • Planning, Budget, and Results
    • Organization Chart
    • EPA History

Breadcrumb

  1. Home
  2. Test Guidelines for Pesticides and Toxic Substances

EDSP Test Guidelines and Guidance Document

The final Endocrine Disruptor Screening Program Test Guidelines are generally intended to meet testing requirements under TSCA, FIFRA and FFDCA to determine if a chemical substance may pose a risk to human health or the environment due to the disruption of the endocrine system.

Group A - EDSP Tier 1 Test Guidelines

890.1100 – Amphibian Metamorphosis (Frog)
890.1150 – Androgen Receptor Binding (Rat Prostate)
890.1200 – Aromatase (Human Recombinant)
890.1250 – Estrogen Receptor Binding
890.1300 – Estrogen Receptor Transcriptional Activation (Human Cell Line HeLa-9903)
890.1350 – Fish Short-Term Reproduction
890.1400 – Hershberger (Rat)
890.1450 – Female Pubertal (Rat)
890.1500 – Male Pubertal (Rat)
890.1550 – Steroidogenesis (Human Cell Line – H295R)
890.1600 – Uterotrophic (Rat)

Group B - EDSP Tier 2 Test Guidelines

890.2100 – Avian Two-Generation Toxicity Test in the Japanese Quail
890.2200 – Medaka Extended One Generation Reproduction Test
890.2300 – Larval Amphibian Growth and Development Assay (LAGDA)
870.3800 - Reproduction and Fertility Effects (2-Gen Repro) 
OECD Test No. 443 - Extended One-Generation Reproductive Toxicity Study 

Guidance Document

Comparative Thyroid Assay

Test Guidelines for Pesticides and Toxic Substances

  • What are Test Guidelines
  • Final Test Guidelines
  • Draft Test Guidelines
  • Master Lists of Test Guidelines
Contact Us About Test Guidelines for Pesticides and Toxic Substances
Contact Us to ask a question, provide feedback, or report a problem.
Last updated on July 30, 2024
  • Assistance
  • Spanish
  • Arabic
  • Chinese (simplified)
  • Chinese (traditional)
  • French
  • Haitian Creole
  • Korean
  • Portuguese
  • Russian
  • Tagalog
  • Vietnamese
United States Environmental Protection Agency

Discover.

  • Accessibility Statement
  • Budget & Performance
  • Contracting
  • EPA www Web Snapshot
  • Grants
  • No FEAR Act Data
  • Plain Writing
  • Privacy
  • Privacy and Security Notice

Connect.

  • Data
  • Inspector General
  • Jobs
  • Newsroom
  • Regulations.gov
  • Subscribe
  • USA.gov
  • White House

Ask.

  • Contact EPA
  • EPA Disclaimers
  • Hotlines
  • FOIA Requests
  • Frequent Questions
  • Site Feedback

Follow.