OIL SPILL EATER II. National Contingency Plan Product Bulletin

Technical Product Bulletin #B-53
Original listing date: AUGUST 26, 1996
REMOVAL DATE: AUGUST 16, 2005
RELISTING DATE: SEPTEMBER 18, 2009
"OIL SPILL EATER II (OSE II)"

I. Name, brand, or trademark
OIL SPILL EATER II (OSE II) Type of Product: Bioremediation Agent (Biological Enzyme Additive [previously listed as a Nutrient Additive])

II. Name, address, and telephone number of manufacturer/CONTACT
OSEI Corporation (Formerly Sky Blue Chems) P.O. Box 515429 Dallas, TX 75251-5429 Phone: (972) 669-3390 E-mail: oseicorp@msn.com Web Site (Mr. Steven Pedigo, Chairman, CEO, Inventor)

III. Name, address, and telephone number of primary distributors
OSEI Corporation (Formerly Sky Blue Chems) P.O. Box 515429 Dallas, TX 75251-5429 Phone: (972) 669-3390 E-mail: oseicorp@msn.com Web Site (Mr. Steven Pedigo, Chairman, CEO, Inventor)

IV. Special handling and worker precautions for storage and field application
1. Flammability: Water-based, non-flammable 2. Ventilation: Needs no ventilation; aqueous-based product; does not emit hazardous vapors 3. Skin and eye contact; protective clothing; treatment in case of contact: OSE II is not a primary dermal irritant. Avoid eye contact, and wear goggles if possible for the spray to come in direct contact with eyes. Facilities for quick and copious eye flushing should be provided and prompt medical attention should be sought if exposure and irritation persists. Protective rubber gloves are suggested during handling. Before mixing the product has a smell of fermentation. The product does not give off any harmful vapors. 4.a. Maximum storage temperature: 120ºF 4.b. Minimum storage temperature: None; OSE II can freeze and thaw without adverse effects 4.c. Optimum storage temperature range: 72ºF 4.d. Temperatures of phase separations and chemical changes: 120ºF

IV. Special handling and worker precautions for storage and field application

1. Flammability: Water-based, non-flammable
2. Ventilation: Needs no ventilation; aqueous-based product; does not emit hazardous vapors
3. Skin and eye contact; protective clothing; treatment in case of contact: OSE II is not a primary dermal irritant. Avoid eye contact, and wear goggles if possible for the spray to come in direct contact with eyes. Facilities for quick and copious eye flushing should be provided and prompt medical attention should be sought if exposure and irritation persists. Protective rubber gloves are suggested during handling. Before mixing the product has a smell of fermentation. The product does not give off any harmful vapors.
4.a. Maximum storage temperature: 120ºF
4.b. Minimum storage temperature: None; OSE II can freeze and thaw without adverse effects
4.c. Optimum storage temperature range: 72ºF
4.d. Temperatures of phase separations and chemical changes: 120ºF

V. Shelf life
OSE II has a recommended shelf life of 5 years. After 5 years at optimum storage temperature, there is an approximate 10% decrease per year in product capability.

VI. Recommended application procedure
1. Application Method: A. Use surface spray apparatus, such as small hand held tanks, back pack, large mixing tanks with mechanical pumping devices, vessels with booms for spraying wide paths, or spray devices on airplanes or helicopters. B. OSE II can be applied by eductor systems from vessels, fire trucks, etc. Set the eductor system to 2% and apply 1 gallon of mixed OSE II to each spilled gallon of hydrocarbon. 2. Concentration/Application Rate: General - OSE II generally takes 3 to 30 minutes to penetrate the molecular walls of hydrocarbons. However, once you spray OSE II on the hydrocarbons, OSE II attaches itself and will eventually engulf the hydrocarbons regardless of where the hydrocarbons may spread on the surface of salt or fresh water. Additionally, once you spray OSE II, the hydrocarbons cannot attach itself to the shoreline, rocks, or any equipment in its path. OSE II breaks down the adhesion properties of hydrocarbons and causes hydrocarbons to float, thereby, eliminating secondary contamination of the water column or any other areas, and holding the contaminated area to the waters surface, the original contaminated area. If OSE II is to be used on ocean spills or on intertidal zones OSE II should be mixed with ocean water. If OSE II is to be used on lakes, rivers, streams, ponds, or on land mix the product with water from a lake, stream, or pond. If you are performing a clean up, make sure the water used to mix with OSE II, and the water used to keep the area saturated, is the type of water normally associated with that area. If you use fresh water in an area normally contacted with salt water or vice versa, the different types of bacteria and competition could occur, not to mention the problems with salinity for fresh water organisms. [Note: Do not mix tap water with OSE II if possible: Chlorine in tap water slows bacterial enhancement] Spills on Water: Dilute each gallon of OSE II with 50 gallons of fresh, brackish, or salt water - depending on the water associated with the area that has been impacted by the spill. Apply OSE II at a ratio of 1 gallon mixed OSE II to each gallon of hydrocarbon spilled. Apply using hand held sprayers, tank sprayers, booms from vessels, helicopters, or airplanes; by spraying the perimeter first then working toward the middle of the spilled area. Next spray the entire surface of the spill. If the spill is very heavy (more than 2 inches thick) it is recommended that OSE II be applied every day until you have met a 1:1 ratio of OSE II and water mixture to spilled oil/hydrocarbons. Use 1 gallon OSE II for every 50 gallons of hydrocarbons. Use 1 drum of OSE II for every 2,750 gallons of hydrocarbons. If you know gallons of hydrocarbons spilled, multiply gallons of hydrocarbons by 0.02 to get amount of OSE II needed [gallons of hydrocarbons x 0.02 = gallons of OSE II]. If you know barrels of crude oil spilled, multiply barrels of crude oil by 0.015 to get drums of OSE II needed [barrels of crude oil x 0.015 = drums of OSE II]. If you do not know gallons of hydrocarbons or barrels of crude oil, multiply size of spill by 0.0023 to get drums of OSE II needed or by 0.12 to get gallons of OSE II needed [(yards long x yards wide x inches thick) x 0.0.0023 = drums of OSE II or (yards long x yards wide x inches thick) x 0.015 = gallons of OSE II]. Intertidal Zone: Mix each 55 gallon drum of OSE II with 2,750 gallons of fresh, brackish, or salt water. The water used is determined by the type of water associated with the site. OSE II should be applied as the tide recedes (if there is a tide) and once the tide comes in the application should cease until the tide recedes again. Additional applications should only be warranted if spill has been allowed time to percolate into the depths of the soil. If there is no tide, but waves have pushed the spill into the intertidal zone, then there will be direct access to the spill at all times. If possible use string or stakes to grid off the beach or intertidal zone area, and then you can calculate how much premixed OSE II to apply to a given area. If unable to grid off an area then calculate how much OSE II to apply and then determine how much premixed OSE II will flow through a nozzle (gallons per minute) then let application technician know how many gallons to apply in a given area and this can be determined by applying product for a certain time period to get the correct amount of OSE II applied to gain the 1:1 ratio. Note: If the intertidal zone is associated with the sea then mix OSE II with salt water. If the spill area is in an area of brackish water then mix OSE II with brackish water. If the intertidal zone is associated with fresh water such as lakes, rivers, streams, ponds, creeks, aquifers, or drinking water wells then use fresh water to mix OSE II. 3. Conditions for Use: OSE II can remediate hydrocarbon-based material including chlorinated hydrocarbons, PCB's, dioxins, and some pesticides. As the age of spilled hydrocarbons increases, the time necessary for bioremediation increases. In general, fresh crude, gasoline of BTEX takes from 72 hours to 30 days to completely bioremediate. Variations of sea water salinity should have no effect, but as long as microbial life can exist, then OSE II will be effective. OSE II bioremediation slows somewhat at temperatures below 40ºF. OSE II however, will continue to work at any liquid water temperature that will sustain microbial life.

VI. Recommended application procedure

1. Application Method:
A. Use surface spray apparatus, such as small hand held tanks, back pack, large mixing tanks with mechanical pumping devices, vessels with booms for spraying wide paths, or spray devices on airplanes or helicopters.
B. OSE II can be applied by eductor systems from vessels, fire trucks, etc. Set the eductor system to 2% and apply 1 gallon of mixed OSE II to each spilled gallon of hydrocarbon.

2. Concentration/Application Rate:
General - OSE II generally takes 3 to 30 minutes to penetrate the molecular walls of hydrocarbons. However, once you spray OSE II on the hydrocarbons, OSE II attaches itself and will eventually engulf the hydrocarbons regardless of where the hydrocarbons may spread on the surface of salt or fresh water. Additionally, once you spray OSE II, the hydrocarbons cannot attach itself to the shoreline, rocks, or any equipment in its path. OSE II breaks down the adhesion properties of hydrocarbons and causes hydrocarbons to float, thereby, eliminating secondary contamination of the water column or any other areas, and holding the contaminated area to the waters surface, the original contaminated area.

If OSE II is to be used on ocean spills or on intertidal zones OSE II should be mixed with ocean water.
If OSE II is to be used on lakes, rivers, streams, ponds, or on land mix the product with water from a lake, stream, or pond.
If you are performing a clean up, make sure the water used to mix with OSE II, and the water used to keep the area saturated, is the type of water normally associated with that area.
If you use fresh water in an area normally contacted with salt water or vice versa, the different types of bacteria and competition could occur, not to mention the problems with salinity for fresh water organisms.

[Note: Do not mix tap water with OSE II if possible: Chlorine in tap water slows bacterial enhancement]

Spills on Water:
Dilute each gallon of OSE II with 50 gallons of fresh, brackish, or salt water - depending on the water associated with the area that has been impacted by the spill. Apply OSE II at a ratio of 1 gallon mixed OSE II to each gallon of hydrocarbon spilled. Apply using hand held sprayers, tank sprayers, booms from vessels, helicopters, or airplanes; by spraying the perimeter first then working toward the middle of the spilled area. Next spray the entire surface of the spill. If the spill is very heavy (more than 2 inches thick) it is recommended that OSE II be applied every day until you have met a 1:1 ratio of OSE II and water mixture to spilled oil/hydrocarbons.

Use 1 gallon OSE II for every 50 gallons of hydrocarbons.
Use 1 drum of OSE II for every 2,750 gallons of hydrocarbons.
If you know gallons of hydrocarbons spilled, multiply gallons of hydrocarbons by 0.02 to get amount of OSE II needed [gallons of hydrocarbons x 0.02 = gallons of OSE II].
If you know barrels of crude oil spilled, multiply barrels of crude oil by 0.015 to get drums of OSE II needed [barrels of crude oil x 0.015 = drums of OSE II].
If you do not know gallons of hydrocarbons or barrels of crude oil, multiply size of spill by 0.0023 to get drums of OSE II needed or by 0.12 to get gallons of OSE II needed [(yards long x yards wide x inches thick) x 0.0.0023 = drums of OSE II or (yards long x yards wide x inches thick) x 0.015 = gallons of OSE II].

Intertidal Zone:
Mix each 55 gallon drum of OSE II with 2,750 gallons of fresh, brackish, or salt water. The water used is determined by the type of water associated with the site. OSE II should be applied as the tide recedes (if there is a tide) and once the tide comes in the application should cease until the tide recedes again. Additional applications should only be warranted if spill has been allowed time to percolate into the depths of the soil.

If there is no tide, but waves have pushed the spill into the intertidal zone, then there will be direct access to the spill at all times. If possible use string or stakes to grid off the beach or intertidal zone area, and then you can calculate how much premixed OSE II to apply to a given area. If unable to grid off an area then calculate how much OSE II to apply and then determine how much premixed OSE II will flow through a nozzle (gallons per minute) then let application technician know how many gallons to apply in a given area and this can be determined by applying product for a certain time period to get the correct amount of OSE II applied to gain the 1:1 ratio.

Note: If the intertidal zone is associated with the sea then mix OSE II with salt water. If the spill area is in an area of brackish water then mix OSE II with brackish water. If the intertidal zone is associated with fresh water such as lakes, rivers, streams, ponds, creeks, aquifers, or drinking water wells then use fresh water to mix OSE II.

3. Conditions for Use:

OSE II can remediate hydrocarbon-based material including chlorinated hydrocarbons, PCB's, dioxins, and some pesticides.
As the age of spilled hydrocarbons increases, the time necessary for bioremediation increases. In general, fresh crude, gasoline of BTEX takes from 72 hours to 30 days to completely bioremediate.
Variations of sea water salinity should have no effect, but as long as microbial life can exist, then OSE II will be effective.
OSE II bioremediation slows somewhat at temperatures below 40ºF. OSE II however, will continue to work at any liquid water temperature that will sustain microbial life.

VII. Toxicity and effectiveness

a. Effectiveness:
Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT OSE II	43,170 40,569 41,730	- - -	11,435 11,785 12,155	- - -
7	CONTROL NUTRIENT OSE II	39,250 34,815 26,316	9.1 14.2 36.9	10,355 9,898 8,072	9.4 16.0 33.6
28	CONTROL NUTRIENT OSE II	35,797 26,507 4,273	17.1 34.7 89.8	9,534 8,938 1,268	16.6 24.2 89.6

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 16.5%
Nutrient: 52.0%
Product: 85.4%

VIII. Microbiological analysis
1. Listing of each component of the total formulation, other than enzymes, by chemical name and percentage by weight: CONFIDENTIAL 2. Enzyme Names: CONFIDENTIAL 3. I.U.B.: CONFIDENTIAL 4. Source of Enzymes: Fermentation process 5. Units: No less than 1% and no more than 50% by weight 6. Specific Gravity: 1.05 7. Optimum Conditions: pH: 7.0 Temperature: 72ºF Salinity Ranges: Fresh water to salt water Maximum and Minimum pH: 3.5 - 8.0 Maximum and Minimum Temperature: 28ºF - 128ºF Maximum and Minimum Salinity Levels - Salinity level above that will support microbial activity will adversely effect OSE II's performance Enzyme Shelf Life: Up to 5 years when properly stored Enzyme Optimal Storage Conditions: 72ºF is optimal, enzyme range is freezing to 120ºF, never leave OSE II in direct sunlight for more than a couple of hours

VIII. Microbiological analysis

1. Listing of each component of the total formulation, other than enzymes, by chemical name and percentage by weight: CONFIDENTIAL
2. Enzyme Names: CONFIDENTIAL
3. I.U.B.: CONFIDENTIAL
4. Source of Enzymes: Fermentation process
5. Units: No less than 1% and no more than 50% by weight
6. Specific Gravity: 1.05
7. Optimum Conditions:

pH: 7.0
Temperature: 72ºF
Salinity Ranges: Fresh water to salt water
Maximum and Minimum pH: 3.5 - 8.0
Maximum and Minimum Temperature: 28ºF - 128ºF
Maximum and Minimum Salinity Levels - Salinity level above that will support microbial activity will adversely effect OSE II's performance
Enzyme Shelf Life: Up to 5 years when properly stored
Enzyme Optimal Storage Conditions: 72ºF is optimal, enzyme range is freezing to 120ºF, never leave OSE II in direct sunlight for more than a couple of hours

IX. Physical properties
NA

X. Analysis for heavy metals, cyanide, and chlorinated hydrocarbons
NA

OIL SPILL EATER II. National Contingency Plan Product Bulletin

Emergency Response