Approved CWA Microbiological Methods for Wastewater and Sewage Sludge

Methods approved under Clean Water Act Section 304(h) and published at 40 CFR Part 136.

• 1600.1: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-β-D-Glucoside Agar (mEI) (pdf) (1020.29 KB, September 2023, EPA-821-R-23-006)
• 1603.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC) (pdf) (957.93 KB, September 2023, EPA-821-R-23-008)
• 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC Medium (pdf) (441.08 KB, September 2014, 821-R-14-009)
• 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using A-1 medium (pdf) (233.6 KB, July 2006, 821-R-06-013)
• 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium (pdf) (282.85 KB, July 2006, 821-R-06-14)