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Exploring ToxCast Data: Downloadable Data

The results after processing through the Pipeline are available on the ToxCast Dashboard, and for most users EPA recommends accessing the data there.

ToxCast Data and Information

  • ToxCast & Tox21 Summary Files. Data for a single chemical endpoint pair for thousands of chemicals and assay endpoints for 20 variables such as the activity or hit call, activity concentrations, whether the chemical was tested in a specific assay, etc.
  • ToxCast & Tox21 Data Spreadsheet. A spreadsheet of EPA's analysis of the chemicals screened through ToxCast and the Tox21 collaboration which includes EPA's activity calls from the  screening of over 1,800 chemicals.
  • ToxCast Data Pipeline R Package. The R computer programming package used to process and model all EPA ToxCast and Tox21 chemical screening data. The files include the R programming package as well as documents that provide overviews of the data analysis pipeline used and the R package. Users will need experience with R to use these files.
  • ToxCast Database (invitroDB):  Database of EPA's analysis of chemicals screened through ToxCast assays, includes a MySQL database and the R package used to process the data.
  • ToxCast & Tox21 Concentration Response Plots. Concentration response plots for all of the ToxCast and Tox21 assays.
  • OECD GD 211 ToxCast Endocrine-Related Assay Documentation. Descriptions and guidelines for ToxCast endocrine-related assays in format outlined by the OECD Guidance Document 211 for  describing non-guideline in vitro test methods. The intent of GD 211 is to harmonize non-guideline, in vitro method descriptions to allow assessment of the relevance of the test method for biological responses of interest and the quality of the data produced. 
  • ToxCast & Tox21 Chemicals Distributed Structure-Searchable Toxicity Database (DSSTox files). Chemical lists and information for 9,403 unique substances (DTXSIDs) and DSSTox standard chemical fields (chemical name; CASRN; structure; etc.). These chemicals have been evaluated through the ToxCast and Tox21 high-throughput screening efforts.
  • ToxCast & Tox21 High-Throughput Assay Information. ToxCast high-throughput assay information including assay annotation user guide, assay target information, study design information and quality statistics on the assays.
  • Standard Laboratory Protocol for Tox21 Assays. Data describing the standard laboratory protocol for Tox21 assays including descriptions of Tox21 assays, protocol for all assays (reference, quality control, procedures and performance) and assay data. 
  • Collaborative Estrogen Receptor Activity Prediction Project Data. Data and supplemental files from CERAPP (A large-scale modeling project) which demonstrated the efficacy of using predictive computational models trained on high-throughput screening data to evaluate thousands of chemicals for estrogen-related activity. CERAPP combined multiple models developed in  collaboration with 17 groups in the United States and Europe to predict ER activity of a common set of 32,464 chemical structures. Quantitative structure-activity relationship models and docking approaches were employed, to build a total of 40 categorical and 8 continuous models for binding, agonist, and antagonist ER activity.
  • High-throughput Screening Data for Estrogen Receptor Model. Estrogen receptor model data from the manuscript titled Integrated Model of Chemical Perturbations of Biological Pathways Using 18 In Vitro High-throughput Screening Assays for the Estrogen Receptor (Judson et al) published in Toxicological Science.
  • High-throughput Screening Data for Androgen Receptor Model. Androgen receptor activity model data.
  • Previous ToxCast Data Releases. Data files from previously published ToxCast data releases. We DO NOT recommend using this data for new analyses, but are providing these files in case users need them for ongoing analyses.
  • Animal Toxicity Studies: Effects and Endpoints. The Toxicity Reference Database (ToxRefDB) provides results form across the thousands of animal toxicity studies including No Effect Level (NEL)/Lowest Effect Level (LEL) and/or No Observed Adverse Effect Level (NOAEL)/Lowest Observed Adverse Effect Level (LOAEL).

ToxCast Results and Processing

Descriptions of how data are processed through can be found on the Results Processing and Analysis.

Links